Electron Microscopy Core

电子显微镜核心

• 批准号: 7280266
• 负责人: THOMAS WALZ
• 金额: $ 160.51万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7280266
• 关键词: Budgets; Clathrin; Collaborations; Complex; Computer software; Computers; Core Facility; Data Collection; Development; Electron Microscopy; Electron Microscopy Facility; Equipment; Future; Grant; Guns; Housing; Image Analysis; Intervention; Location; Methods; Microscope; Molecular; Operative Surgical Procedures; Postdoctoral Fellow; Purpose; Research; Resolution; Spiders; Staging; Staining method; Stains; Standards of Weights and Measures; Structure; Students; Three-Dimensional Image; Three-Dimensional Imaging; Time; Training; Universities; Wages; Work; cluster computing; experience; human disease; instrument; instrumentation; medical schools; outreach; particle; programs; structural biology; tool

The EM core of this Program Project has two related purposes - to support instrumentation essential for the research proposed in the four projects of this grant and to facilitate application of the methods being developed in these projects to collaborative and independent efforts. The principal components of the core are the electron microscopy facilities at Harvard Medical School (HMS) and at Brandeis University. The HMS facility will house four FBI microscopes: a Tecnai F20, a Tecnai T12, a CM10, and a Polara F30. The F30, the F20, and the T12 microscopes are equipped with cryo-stages; the F30 and the F20 have field-emission guns. The Brandeis facility also houses four FBI instruments: a Morgagni M268, and EM20, a CM12, and a Tecnai F30. The last three have cryo-stages; the F30 has a field-emission gun and an energy filter. The core supports the operation of these microscopes and the salary of a facilities manager at each location, as well as necessary ancillary equipment. The core also has a computational component. It maintainsand upgrades all standard EM software, including program suites such as FREALIGN, IMAGIC, and SPIDER, through a structural biology computational grid (SBGrid) established by the Center for Molecular and Cellular Dynamics at HMS. Finally, by providing EM and computational facilities, the core allows the P.I.'s of these projects to collaborate with other groups at various institutionsand to train postdoctoral fellows and students in the relevant methods. Relevance: Electron microscopy is the key experimental tool that bridges between low- and high-resolution structural methods. Large assemblies will be targets of future interventions in human disease, and electron microscopy will be central for relating structure and function of these complexes

该计划项目的EM核心有两个相关目的 - 支持仪器对 该赠款的四个项目中提出的研究并促进了正在开发的方法 在这些项目中进行协作和独立的努力。核心的主要成分是电子 哈佛医学院（HMS）和布兰代斯大学的显微镜设施。 HMS设施将 房屋四个FBI显微镜：Tecnai F20，Tecnai T12，A CM10和Polara F30。 F30，F20和 T12显微镜配备了冷冻阶段； F30和F20具有现场排放枪。布兰代斯 设施还设有四种FBI仪器：Morgagni M268和EM20，A CM12和Tecnai F30。最后 三个有冷冻阶段； F30具有现场发射枪和一个能量过滤器。核心支持操作 这些显微镜和设施经理在每个位置的薪水以及必要的辅助 设备。核心还具有计算组件。它维护和升级所有标准EM软件， 通过结构生物学，包括Frealign，Imagic和Spider等程序套件 由HMS分子和细胞动力学中心建立的计算网格（SBGRID）。最后， 通过提供EM和计算设施，核心允许这些项目的P.I.与 各个机构的其他团体和以相关方法培训博士后研究员和学生。 相关性：电子显微镜是桥接低分辨率和高分辨率之间的关键实验工具 结构方法。大型组合将是人类疾病的未来干预措施的目标，电子 显微镜将是关联这些复合物的结构和功能的中心