Tissue Culture Biomechanical Core

组织培养生物力学核心

基本信息

批准号：
7407794
负责人：
VISWANATHAN NATARAJAN
金额：
$ 22.41万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-02-01 至 2013-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7407794
关键词：
Actins Adherens Junction Annona muricata coronin Atomic Force Microscopy Biomechanics CD44 gene Code Computer software Core Facility Cultured Cells Cytoskeletal Proteins Cytoskeleton Endothelial Cells Engineering Ensure Environment Equipment Focal Adhesions Fostering Functional disorder Genetic Polymorphism Genetically Engineered Mouse Goals Growth Factor Human Human Resources Hyperoxia Individual Inflammatory Laboratory Personnel Lung MAP Kinase Gene MYLK gene Measurement Measures Mechanical Stress Mediating Membrane Microdomains Microscopic Modeling Mus NADPH Oxidase Pathology Pathway interactions Permeability Phospholipase D Phosphorylation Phosphotransferases Process Protein Isoforms Protein Overexpression Proteins Proto-Oncogene Protein pp60 (c-src)RNA Splicing Regulation Research Personnel Resources Role Services Signal Transduction Stretching Structure Techniques Tight Junctions Training Transgenic Organisms Variant Vascular Endothelial Growth Factors Work caveolin 1 coronin protein design edg-1 Protein human EMS1 protein in vivo instrumentation lung injury paxillin polymerization programs protein kinase A kinase pulmonary artery endothelial cell research study rho software systems tissue culture

项目摘要

The Tissue Culture/Biomechanical Core (Core B) will provide critical support to Program Project Investigators while serving as a resource center and mechanism of integration between the five Projects. The Core facility will serve as a centralized resource unit providing laboratory personnel, expertise, and equipment to meet all human and murine endothelial cell needs for projects in the program. As all the five projects and Core D share a common theme of endothelial barrier regulation by the cytoskeleton, the major emphasis of this core will be to culture primary human pulmonary artery endothelial cells (HPAECs), human lung microvascular endothelial cells (HLMVECs), and isolate lung microvascular endothelial cells from various transgenic and genetically-altered murine models. The core has well trained personnel to process all the cell culture work and isolate lung microvascular ECs from murine models. The expertise and training we have developed during the last two cycles of this program project have defined the most effective mechanisms for culturing endothelial cells to assess permeability changes and/or pathologies. Another critical function of this Core will be facilitate experiments with cyclic stretch and atomic force microscopy (Core D) using dedicated instrumentation, computers and software systems. The close proximity of the tissue culture, biophysical and atomic force microscopy facilities will foster interaction between the investigators to develop and design experiments and interpret the reults. This will provide a unique environment where cell culture/biomechanical models and microscopic techniques will be refined and tailored to specific scientific needs of the projects. Thus, the tissue culture/biomechanical core facility is an integral component of the program project that ensures the most effective and economical use of the resources and expertise to meet the goals for each project.

组织培养/生物力学核心（核心 B）将为计划项目提供关键支持调查人员同时充当五个项目之间的资源中心和整合机制。核心设施将作为一个集中资源单位，提供实验室人员、专业知识和满足该计划项目的所有人类和小鼠内皮细胞需求的设备。正如所有五个项目和核心 D 有一个共同的主题，即细胞骨架对内皮屏障的调节，这是主要的该核心的重点是培养原代人肺动脉内皮细胞（HPAEC）、人肺微血管内皮细胞（HLMVEC），并从其中分离出肺微血管内皮细胞各种转基因和基因改变的小鼠模型。核心拥有训练有素的人员来处理所有细胞培养工作并从小鼠模型中分离出肺微血管 EC。我们的专业知识和培训在该计划的最后两个周期中制定的项目已经确定了最有效的培养内皮细胞以评估渗透性变化和/或病理的机制。其他该核心的关键功能将促进循环拉伸和原子力显微镜实验（核心D）使用专用仪器、计算机和软件系统。距离很近组织培养、生物物理和原子力显微镜设施将促进研究人员开发和设计实验并解释结果。这将提供一个独特的细化细胞培养/生物力学模型和显微技术的环境根据项目的具体科学需求量身定制。因此，组织培养/生物力学核心设施是一个该计划项目的一个组成部分，确保最有效和最经济地利用资源和专业知识来实现每个项目的目标。