Ariol SL-50 Workstation and Review Stations

Ariol SL-50 工作站和检查站

• 批准号: 6877240
• 负责人: MARY Kay WASHINGTON
• 金额: $ 33.79万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877240
• 关键词: biomedical automation; biomedical equipment purchase; biomedical resource; clinical chemistry; computer data analysis; computer network; computer program /software; cytodiagnosis; diagnosis quality /standard; exfoliative cytology; fluorescent dye /probe; fluorescent in situ hybridization; immunocytochemistry; molecular /cellular imaging

Investigation of biomarkers of pre-neoplasia as well as markers for prognosis and response to individualized therapies is increasingly important in translational research, a major focus of Vanderbilt University School of Medicine. One recent advance relevant to these translational efforts is the development of tissue microarrays (TMAs), which allow high through-put analysis of expression of numerous markers as determined by immunohistochemistry and immunofluorescence. However, the utility of TMAs is limited by the subjective semi-quantitative nature of the data analysis by individual pathologists. We are requesting funds to purchase an Ariol SL-50 Automated Cellular Scanning Quantitation System to overcome these limitations. This system will obtain digitized images of stained slides at various magnifications, including different types of stains, counterstains, immunohistochemical chromagens, and multiple fluorescent probes. Because the Ariol SL-50 system is automated and can scan in 50 slides overnight, facilitation of analysis of multiple slides without human intervention is possible. The images are easily reviewable by a consulting pathologist to confmn the veracity of interpretation. The most important characteristic of the system is the ability to quantify staining using varying calculations including the area of staining as a percent of total tissue area, staining per cell and number of stained cells and to differentiate patterns of intracellular staining among nuclear, cytoplasmic and plasma membrane locations. Operation of the instrument will be supervised by Dr. Washington, who will also oversee maintenance. Service contract fees will be offset by charging a small user fee per slide. Major users are primarily investigators from the VUMC Lung, GI, and Breast SPOREs, the Skin Diseases Research Center, and the Digestive Diseases Research Center. Use of the system and prioritization of access will be under the oversight of the Utilization Committee. The presence of this instrument will allow us to embark on the large-scale translational biomarker studies that are required for evaluation of diagnostic and prognostic markers. An additional benefit is the ease of data sharing among groups, facilitating interactions among investigators and promoting inter-SPORE collaborations.

肿瘤前期生物标志物以及预后和反应标志物的研究 个体化治疗在转化研究中变得越来越重要，这是范德比尔特大学医学院的一个主要关注点。与这些转化工作相关的最新进展是组织微阵列（TMA）的开发，它可以对通过免疫组织化学和免疫荧光测定的多种标记物的表达进行高通量分析。然而，TMA 的实用性受到个别病理学家数据分析的主观半定量性质的限制。我们正在申请资金购买 Ariol SL-50 自动细胞扫描定量系统，以克服这些限制。该系统将以各种放大倍数获得染色载玻片的数字化图像，包括 不同类型的染色剂、复染剂、免疫组织化学显色剂和多种荧光探针。由于 Ariol SL-50 系统是自动化的，可以在一夜之间扫描 50 张载玻片，因此无需人工干预即可促进多张载玻片的分析。咨询病理学家可以轻松查看这些图像，以确认解释的准确性。该系统最重要的特征是能够使用不同的计算来量化染色，包括染色面积占总组织面积的百分比、每个细胞的染色和染色细胞的数量，并区分细胞核、细胞质和血浆之间的细胞内染色模式膜位置。该仪器的操作将由华盛顿博士监督，他还将监督维护。服务合同费用将通过每张幻灯片收取少量使用费来抵消。主要用户主要是来自 VUMC 肺、胃肠道和乳腺孢子、皮肤病研究中心和消化系统疾病研究中心的研究人员。系统的使用和访问的优先顺序将受到使用委员会的监督。该仪器的存在将使我们能够开展评估诊断和预后标志物所需的大规模转化生物标志物研究。另一个好处是群体之间可以轻松共享数据，促进研究人员之间的互动并促进 SPORE 之间的合作。