Core 1: Tissue Pathology and Cellular Analysis

核心 1：组织病理学和细胞分析

基本信息

批准号：
10443608
负责人：
MARY Kay WASHINGTON
金额：
$ 31.13万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-09 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10443608
关键词：
Academic Medical Centers Animal Cancer Model Antibodies Basic Science Behavior Bioinformatics Biological Assay Biological Process Biometry Cancer Center Cancer Science Cells Collaborations Collection Colorectal Cancer Colorectal Neoplasms Complement Computer Analysis Confidentiality of Patient Information Consult Consultations Cooperative Human Tissue Network Custom Cytometry Data Data Analyses Databases Diagnosis Digestive System Disorders Disease Early Diagnosis Ensure Epithelial Epithelial Cells Evaluation Formalin Freezing Gastrointestinal Neoplasms Gene Expression Generations Histologic Histology Histopathology Human Image Immunofluorescence Immunologic Immunohistochemistry Informatics Informed Consent Infrastructure Malignant neoplasm of gastrointestinal tract Mission Molecular Morphology Mus Normal tissue morphology Paraffin Embedding Pathology Patient Care Patients Play Pre-Clinical Model Preparation Prevention Process Protein Analysis Publishing Quality Control Reagent Research Research Activity Research Personnel Resource Sharing Role Running Sampling Services Signal Transduction Specimen Technology The Vanderbilt-Ingram Cancer Center at the Vanderbilt University Time Tissue Banks Tissue Microarray Tissue Sample Tissues Translational Research Validation Washington Work biobank cancer biomarkers cellular imaging colorectal cancer treatment cost effective data de-identification data quality digital flexibility human disease image processing instrument laser capture microdissection molecular imaging mouse model next generation novel operation protein expression repository screening single cell analysis single-cell RNA sequencing synergism tool translational goal

项目摘要

Project Summary/Abstract: Tissue Pathology and Cellular Analysis Core The Translational Pathology and Cellular Analysis (TPCA) Core, will play a central role in the translational research mission of this SPORE, providing essential support for all projects by providing annotated human biospecimens and research histology services. It includes with a single-cell analysis subcore under the direction of Dr. Ken Lau. This subcore will enable technologies for quantifying and analyzing protein expression at a multiplex, single-cell level, greatly augmenting the histopathology capabilities of the TPCA on both FFPE, fresh, and frozen tissue samples. While the primary mission of the TPCA is state-of-the-art immunohistochemistry, research pathology and biorepository services, the single-cell analysis subcore will offer three cutting-edge technologies: inDrop single-cell RNA-Seq, MxIF, and DISSECT-CyTOF, and their associated computational analyses. The single-cell analysis subcore will offer services, from the preparation and processing of samples, the generation and verification of epithelial-specific reagents, screening and validation of targets, and finally, computational data analysis. Single-cell data will be synthesized and presented to investigators in adaptable formats for presentation and/or downstream analysis. A specific strength of this subcore in relation to the GI SPORE is that Dr. Ken Lau's research focus is on GI disorders and his expertise will greatly synergize with the research of GI neoplasms. We can offer highly specific molecular assays that can flexibly query a wide range of biological processes including the evaluation of signaling, cell identity, function, and behavior, all within a single cell. The TPCA will partner with other established tissue collection and immunohistochemistry shared resources, such as the Vanderbilt University Medical Center Translational Pathology Shared Resource and the Digital Histology Shared Resource, to provide exceptional services to the GI SPORE in a highly cost-effective manner. Close collaboration with the Biostatistics and Bioinformatics Core will provide the TPCA and GI SPORE with robust informatics and database tools for collection, distribution, and annotation of biospecimens in the GI SPORE repository. Dr. Washington and Dr. Lau have developed synergistic working relationships with imaging services under the direction of Dr. Charles Manning and these will be maintained between the TPCA and the Mouse and Human Molecular Imaging Core.

项目摘要/摘要：组织病理学和细胞分析核心转化病理学和细胞分析（TPCA）核心将在转化中发挥核心作用该 SPORE 的研究任务，通过提供带注释的人类为所有项目提供必要的支持生物样本和研究组织学服务。它包括在指导下的单细胞分析子核心刘健博士。该子核心将使定量和分析蛋白质表达的技术成为可能多重、单细胞水平，大大增强了 TPCA 在 FFPE、新鲜、和冷冻组织样本。虽然 TPCA 的主要任务是最先进的免疫组织化学，研究病理学和生物样本库服务，单细胞分析子核心将提供三种尖端技术技术：inDrop 单细胞 RNA-Seq、MxIF 和 DISSECT-CyTOF 及其相关计算分析。单细胞分析子核心将提供从样品制备和处理、上皮特异性试剂的生成和验证、靶标的筛选和验证，最后，计算数据分析。单细胞数据将被合成并以适应性强的方式呈现给研究人员用于演示和/或下游分析的格式。该子核心相对于 GI 的特定强度 SPORE 是 Ken Lau 博士的研究重点是胃肠道疾病，他的专业知识将与胃肠道肿瘤的研究。我们可以提供高度特异性的分子检测，可以灵活地查询各种生物过程，包括信号传导、细胞身份、功能和行为的评估，全部在一个单一的细胞。 TPCA 将与其他已建立的组织收集和免疫组织化学共享资源合作，例如范德比尔特大学医学中心转化病理学共享资源和数字组织学共享资源，以极具成本效益的方式为 GI SPORE 提供卓越的服务。与生物统计学和生物信息学核心的密切合作将为 TPCA 和 GI SPORE 提供用于收集、分发和注释 GI 中生物样本的强大信息学和数据库工具孢子存储库。华盛顿博士和刘博士在成像领域建立了协同工作关系在 Charles Manning 博士的指导下提供服务，这些服务将在 TPCA 和小鼠和人类分子成像核心。