Robotics Extraction System/HPLC for Shared Resource Lab

用于共享资源实验室的机器人提取系统/HPLC

• 批准号: 6876962
• 负责人: MARK C CHAPPELL
• 金额: $ 11.93万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876962
• 关键词: analytical method; biological products; biomedical automation; biomedical equipment purchase; blood; cells; growth media; high performance liquid chromatography; physical separation; robotics; sample collection; tissue /cell preparation; urine

DESCRIPTION (provided by applicant): The grant application requests funds for the purchase of a Gilson SPE 215 automated liquid handling and solid phase extraction/high pressure chromatography system to facilitate the processing and analysis of biological samples by the Hypertension Center Assay Core laboratory at the Wake Forest University School of Medicine (WFUSM). Currently all sample handling and extractions are performed manually by the Hypertension Core personnel, under the supervision of Dr. Bridget Brosnihan. Given the large amount of samples and increasing demand for different RIA or ELISA procedures in various tissues, blood, urine and cell cultures that all require extensive extraction procedures prior to analysis, an automated handling and extraction system is necessary to maintain the precession, efficiency and output of the Hypertension Core Laboratory. Furthermore, the requested instrument allows for direct injection of extracted samples into a high performance liquid chromatography system (HPLC) for sample and assay validation. Although not routinely performed for each sample, HPLC verification of either individual or pooled samples ensures sample identity in immunological analysis performed particularly when combined with extensive extraction methods. This core facility provides support for 15 NIH-funded investigators holding 17 NIH grants and a P01 with 5 individual projects; over 90% of the samples submitted by investigators at WFUSM requiring this equipment are funded by NIH. The Hypertension Core Laboratory offers a low cost, multi-assay facility to investigators in a convenient on-campus setting. The addition of the state-of-the-art sampling/extraction/HPLC system will allow the Hypertension Core Laboratory to meet the increasing sample load and reduce the delivery time of data output to investigators. Therefore, the combination of high quality instrumentation, very-experienced personnel and solid financial support will ensure all NIH-funded researchers will benefit in time, quantity and quality with the acquisition of the proposed instrumentation.

说明（由申请人提供）：拨款申请要求提供资金购买 Gilson SPE 215 自动液体处理和固相萃取/高压色谱系统，以促进高血压中心检测核心实验室对生物样本的处理和分析。维克森林大学医学院 (WFUSM)。目前，所有样本处理和提取均由高血压核心人员在 Bridget Brosnihan 博士的监督下手动进行。鉴于样本量巨大，并且各种组织、血液、尿液和细胞培养物中对不同 RIA 或 ELISA 程序的需求不断增加，在分析之前都需要进行大量的提取程序，因此需要一个自动化处理和提取系统来维持进动、效率和稳定性。高血压核心实验室的产出。此外，所需的仪器允许将提取的样品直接注射到高性能液相色谱系统（HPLC）中以进行样品和测定验证。尽管不是对每个样品进行常规操作，但对单个或混合样品进行 HPLC 验证可确保在免疫分析中进行样品同一性，特别是与广泛的提取方法相结合时。该核心设施为 15 名 NIH 资助的研究人员提供支持，这些研究人员拥有 17 项 NIH 拨款和 5 个单独项目的 P01； WFUSM 的研究人员提交的需要该设备的样本中 90% 以上都是由 NIH 资助的。高血压核心实验室在便利的校园环境中为研究人员提供低成本、多检测设施。添加最先进的采样/提取/高效液相色谱系统将使高血压核心实验室能够满足不断增加的样本量，并减少向研究人员输出数据的交付时间。因此，高质量的仪器、经验丰富的人员和坚实的财政支持相结合，将确保所有 NIH 资助的研究人员都能在购买拟议仪器后在时间、数量和质量上受益。