Zeiss Axiovert 200-M for Time-Lapse Microscopy

用于延时显微镜的 Zeiss Axiovert 200-M

• 批准号: 6877463
• 负责人: Peter N Devreotes
• 金额: $ 17.36万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877463
• 关键词: biomedical equipment purchase; charge coupled device camera; microscopy

DESCRIPTION (provided by applicant): This is an application for a Zeiss inverted microscope with precision motorized stage and sensitive CCD camera for time-lapse, live-cell imaging. The microscope will be used as a dedicated station for constant-focus time-lapse imaging with DIG and fluorescence optics. The precision AS) motorized piezo microscope stage and the Zeiss environmental control system will allow us to image cells at constant focus under physiological conditions for hours or days at a time. This stage will also be used to prepare time-lapse sequences of cells at different locations during the same imaging session. The sensitive Photometrics Cascade CCD camera will significantly reduce photobleaching and maintain cell viability. This application is based on a critical need to monitor cell responses over time by time-lapse recording and will serve a large NIH-funded community.

描述（由申请人提供）：这是蔡司倒置显微镜的申请，配有精密电动载物台和灵敏的 CCD 相机，用于延时活细胞成像。该显微镜将用作使用 DIG 和荧光光学器件进行恒焦延时成像的专用站。精密 AS) 电动压电显微镜载物台和蔡司环境控制系统将使我们能够在生理条件下连续数小时或数天对细胞进行持续聚焦的成像。该阶段还将用于在同一成像过程中准备不同位置的细胞延时序列。灵敏的 Photometrics Cascade CCD 相机将显着减少光漂白并保持细胞活力。该应用程序基于通过延时记录监测细胞随时间变化的反应的迫切需求，并将为 NIH 资助的大型社区提供服务。