Percutaneous Devices Permitting Skin Cell Attachment

允许皮肤细胞附着的经皮装置

• 批准号: 7415026
• 负责人: JOHN Everett OLERUD
• 金额: $ 49.23万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-12 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7415026
• 关键词: Adhesions; Air; Animals; Biocompatible Materials; Biomedical Engineering; Blood; Catheters; Cell Adhesion; Cell Culture System; Cell-Matrix Junction; Cells; Chemicals; Chemistry; Cutaneous; Dermal; Detection; Devices; Dialysis procedure; Electron Microscopy; Endothelial Cells; Engineering; Evaluation; Event; Fibroblasts; Goals; Healed; Human; Image; Immobilization; Immunohistochemistry; Implant; In Situ Hybridization; In Vitro; Infection; Interdisciplinary Study; Light; Limb structure; Marketing; Medical Device; Methods; Microbial Biofilms; Modeling; Molecular; Molecular Biology; Morphology; Mus; Newborn Infant; Object Attachment; Organ Culture Techniques; Patient Care; Pharyngeal structure; Physical Dialysis; Play; Post-Translational Protein Processing; Process; Prosthesis; Proteins; Research; Research Personnel; Risk; Role; Sampling; Skin; Stream; Surface; Testing; United States National Institutes of Health; Venous; base; biomaterial interface; chemotherapy; cost; glucose sensor; healing; implantation; in vitro Model; in vivo; in vivo Model; innovation; keratinocyte; light microscopy; mortality; multi-photon; penis foreskin; poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; prevent; research clinical testing; response; seal; size; wound

DESCRIPTION (provided by applicant): Percutaneous devices play an essential role in patient care. However, the risks of infections associated with penetrating the skin are not inconsequential. Percutaneous devices are used for such diverse functions as dialysis, chemotherapy, attachments for prosthetic limbs and percutaneous sensors (glucose). Central venous catheters alone are associated with an estimated 80,000 to 250,000 blood stream infections annually, with an associated mortality of 12%-25% for each infection and a cost of >$28,000 per episode. The goal of this research is to create an interface that promotes epidermal and dermal cell integration with the surface of percutaneous medical devices. We believe that healing the cutaneous wound with cellular integration into percutaneous medical devices will provide a seal preventing bacterial attachment and subsequent biofilm formation. This innovation may prevent the most common cause of serious complications associated with these devices. Consistent with The NIH Roadmap, the proposed research will be conducted by an interdisciplinary research team including, skin biologists, researchers from two NSF Engineering Research Centers, one with expertise in advanced biomaterials, the other with expertise in the study of biofilm, as well as an industrial partner that develops and markets percutaneous devices for intravascular access. We will carry out the following Specific Aims: 1. Develop and characterize biomaterials that permit attachment of skin cells where percutaneous devices penetrate the skin. 2. Study the cellular and molecular events of the in vitro and in vivo skin responses (biointegration) to optimized biomaterials. 3. Evaluate bacterial attachment and biofilm formation as a function of skin/biomaterial integration.

描述（由申请人提供）：经皮装置在患者护理中发挥着重要作用。然而，与穿透皮肤相关的感染风险并非无关紧要。经皮装置用于多种功能，例如透析、化疗、假肢附件和经皮传感器（葡萄糖）。据估计，每年仅中心静脉导管就与 80,000 至 250,000 起血流感染相关，每次感染的相关死亡率为 12%-25%，每次感染的费用超过 28,000 美元。这项研究的目标是创建一个界面，促进表皮和真皮细胞与经皮医疗设备表面的整合。我们相信，通过细胞整合到经皮医疗器械中来治愈皮肤伤口将提供密封，防止细菌附着和随后的生物膜形成。这项创新可以防止与这些设备相关的严重并发症的最常见原因。根据 NIH 路线图，拟议的研究将由跨学科研究团队进行，其中包括皮肤生物学家、来自两个 NSF 工程研究中心的研究人员，其中一个具有先进生物材料的专业知识，另一个具有生物膜研究的专业知识，以及开发和销售用于血管内通路的经皮设备的工业合作伙伴。我们将实现以下具体目标： 1. 开发并表征生物材料，使皮肤细胞能够附着在经皮装置穿透皮肤的地方。 2. 研究优化生物材料的体外和体内皮肤反应（生物整合）的细胞和分子事件。 3. 评估细菌附着和生物膜形成作为皮肤/生物材料整合的功能。

项目成果

Quantifying the effect of pore size and surface treatment on epidermal incorporation into percutaneously implanted sphere-templated porous biomaterials in mice.

• DOI: 10.1002/jbm.a.33125
• 发表时间: 2011-09-15
• 期刊: Journal of biomedical materials research. Part A
• 作者: Underwood RA;Usui ML;Zhao G;Hauch KD;Takeno MM;Ratner BD;Marshall AJ;Shi X;Olerud JE;Fleckman P
• 通讯作者: Fleckman P