External Tufted Cells Coordinate Olfactory Bulb Activity

外部簇状细胞协调嗅球活动

• 批准号: 7404544
• 负责人: ABDALLAH M HAYAR
• 金额: $ 26.58万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7404544
• 关键词: Action Potentials; Anatomy; Apical; Appendix; Area; Axon; Brain region; Cells; Characteristics; Chemicals; Chromosome Pairing; Cloning; Code; Computer information processing; Dendrites; Dendrodendritic Synapse; Devices; Discrimination; Effectiveness; Electrical Synapse; Elements; Exhibits; Feedback; Fire - disasters; Frequencies; Gap Junctions; Glutamate Receptor; Glutamates; Interneurons; Invaded; Knowledge; Label; Maps; Mediating; Membrane Potentials; Microspheres; Modeling; Neurons; Odorant Receptors; Odors; Olfactory Nerve; Olfactory Receptor Neurons; Output; Pathway interactions; Pattern; Peripheral; Play; Population; Positioning Attribute; Presynaptic Terminals; Process; Property; Recurrence; Research Personnel; Rodent; Role; Sensory; Sensory Process; Series; Shapes; Signal Transduction; Site; Source; Staging; Stimulus; Synapses; Synaptic Transmission; Synaptic plasticity; System; Techniques; Testing; Time; Whole-Cell Recordings; Work; extracellular; granule cell; insight; interest; olfactory bulb; olfactory receptor; patch clamp; postsynaptic; programs; relating to nervous system; research study; transmission process

DESCRIPTION (provided by applicant): Oscillations or cycling discharges of neurons provide a clock or impose a "tempo" for various kinds of information processing. We recently found that olfactory bulb ET cells are endowed with spontaneous rhythmic bursting that persists and becomes more regular in the presence of fast synaptic blockers. Using simultaneous whole-cell recording from pairs of juxtaglomerular (JG) neurons, we were the first to discover that membrane potential oscillations and spontaneous bursting activity are highly correlated in external tufted (ET) cells associated with the same glomeruli. This synchronous activity occurs at theta frequency (1-8 Hz), the same frequency that characterizes investigative sniffing in rodents. Therefore, synchronous ET cell bursting may play an important role in olfactory coding and in regulating the induction of synaptic plasticity at the first input stage of the main olfactory bulb. In this proposal, we hypothesize that ET cells coordinate the activity of other olfactory bulb neurons and may play a role of a pattern generator of the olfactory bulb network. In particular, this project will assess the functional roles of synaptic and non-synaptic interactions in establishing correlated activity between ET cells and other neurons including interneurons and output neurons. We will use dual patch clamp and extracellular recording techniques and cross-correlation analysis to test the following hypotheses: (i) ET cell bursting activity is shaped by feedback dendrodendritic inhibition from PG cells, (ii) ET cells in neighboring glomeruli have correlated activity that is mediated by short axon cells, (iii) ET cells coordinate the activity of other output neurons, tufted and mitral cells, via chemical and electrical synapses. Taken together, the experiments proposed in this study will provide important, new insights into the intrinsic synaptic organization of the glomeruli and the role of glomerular circuitry in olfactory coding.

描述（由申请人提供）：神经元的振荡或循环放电提供时钟或为各种信息处理施加“速度”。我们最近发现，嗅球ET细胞被赋予自发的节奏爆发，在存在快速突触阻滞剂的情况下持续存在并变得更加规律。使用对近后胶质性（JG）神经元对的全细胞记录，我们是第一个发现膜电位振荡和自发爆发活性在与同一肾小球相关的外部簇状（ET）细胞中高度相关的。这种同步活性发生在theta频率（1-8 Hz）上，与啮齿动物中的调查性嗅探相同。因此，同步ET细胞爆发可能在嗅觉编码和调节主要嗅球的第一个输入阶段的突触可塑性方面起重要作用。在此提案中，我们假设ET细胞协调其他嗅球神经元的活性，并可能在嗅球网络的模式发生器中起作用。特别是，该项目将评估突触和非突触相互作用在建立ET细胞与其他神经元之间相关活性（包括中神经元和输出神经元）之间的相关活性中的功能作用。 We will use dual patch clamp and extracellular recording techniques and cross-correlation analysis to test the following hypotheses: (i) ET cell bursting activity is shaped by feedback dendrodendritic inhibition from PG cells, (ii) ET cells in neighboring glomeruli have correlated activity that is mediated by short axon cells, (iii) ET cells coordinate the activity of other output neurons, tufted and mitral cells, via化学和电突触。综上所述，本研究提出的实验将为肾小球的内在突触组织提供重要的新见解，以及肾小球电路在嗅觉编码中的作用。