Mycology Research Unit: In Vivo Induced Fungal Antigens

真菌学研究单位：体内诱导真菌抗原

• 批准号: 7635201
• 负责人: M. Hong Thi NGUYEN
• 金额: $ 50.19万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-15 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7635201
• 关键词: Mycology; Research; Unit; Vivo; Induced

DESCRIPTION (provided by applicant): The Mycology Research Unit: In Vivo Induced Fungal Antigens Program Project consists of four interrelated research projects and a Clinical Resource Core organized around the identification of C. albicans and A. fumigatus proteins expressed during human infections. The unifying hypothesis of the Program is that selected in vivo induced proteins contribute to the pathogenesis of invasive fungal infections and have potential applications as vaccine, diagnostic and therapeutic targets. The Core, run by Dr. Wingard (University of Florida; UF) will collect sera and tissue samples from patients with C. albicans and A. fumigatus infections. The sera will be used extensively in each of the projects. In Project 1 (Candida antigens involved in site-specific virulence), Dr. Nguyen (UF) will study in vivo induced antigens that she and Dr. Clancy identified by screening a genomic expression library with sera from patients with candidiasis. The contributions of the in vivo induced antigens to pathogenesis will be assessed in models of mucosal and systemic candidiasis. She will also characterize the humoral immune response against recombinant antigens in the serum of patients with candidiasis and controls. In Project 2 (A proteomic approach to host humoral immune response), Dr. Liu (UC-Irvine) will construct C. albicans cell wall protein and blood-induced protein arrays and probe them with sera from patients who survived or died from candidemia. Her goal is to identify immunogenic antigens associated with good prognosis. In Project 3 (Candida in vivo expressed protein as a mannan carrier), Dr. Cutler (TRIC,La) will use cell wall antigens identified in Projects 1 and 2 as carrier proteins in a conjugate mannan vaccine against hematogenous candidiasis. Finally, in Project 4 (Identification of In vivo Induced A. fumigatus Antigens), Dr. Clancy (UF) will adapt the serum-based screening that he and Dr. Nguyen developed to screen A. fumigatus expression libraries. In vivo induced A. fumigatus antigens can then be studied using our investigations of C. albicans antigens as models.

描述（由申请人提供）：真菌学研究部门：体内诱导的真菌抗原计划项目由四个相互关联的研究项目和一个围绕人类感染期间表达的白色念珠菌和烟曲霉蛋白质组织的临床资源核心组成。该程序的统一假设是，选定的体内诱导蛋白有助于侵入性真菌感染的发病机理，并具有潜在的应用作为疫苗，诊断和治疗靶标。由Wingard博士（佛罗里达大学； UF）运营的核心将收集白色念珠菌和富米曲霉感染患者的血清和组织样品。血清将在每个项目中广泛使用。在项目1（参与特定地点毒力的念珠菌抗原）中，Nguyen博士（UF）将研究体内诱导的抗原，她和Clancy博士通过筛选来自念珠菌患者的血清的基因组表达文库来鉴定出来。体内诱导的抗原对发病机理的贡献将在粘膜和系统性念珠菌病模型中评估。她还将表征针对念珠菌病和对照患者血清中重组抗原的体液免疫反应。在项目2（一种蛋白质组学的宿主免疫反应方法）中，Liu博士（UC-IRVINE）将构建白色念珠菌细胞壁蛋白和血液诱导的蛋白阵列，并用来自念珠菌生存或死亡的患者的血清探测它们。她的目标是确定与良好预后相关的免疫原性抗原。在项目3（体内念珠菌表示蛋白质为曼南载体）中，卡特勒博士（Tric，La）将使用项目1和2中鉴定的细胞壁抗原在结合曼南南疫苗中针对血源性念珠菌病的载体蛋白作为载体蛋白。最后，在项目4（识别体内诱导的烟曲霉抗原）中，Clancy博士（UF）将适应他和Nguyen博士开发的基于血清的筛选，以筛选A. fumigatus表达文库。然后，可以使用我们对白色念珠菌抗原作为模型的研究来研究体内诱导的烟曲霉抗原。