2008 Proprotein Processing, Trafficking & Secretion

2008 年前蛋白加工、贩运

基本信息

批准号：
7536669
负责人：
RICHARD E MAINS
金额：
$ 1.5万
依托单位：
GORDON RESEARCH CONFERENCES
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7536669
关键词：
Alzheimer&apos s Disease Anabolism Area Biochemical Genetics Biological Cells Complex Diabetes Mellitus Discipline Disease Drug Addiction Endocrine Endopeptidases Enzymatic Biochemistry Enzymes Etiology Exocytosis Faculty Financial Support Foundations Golgi Apparatus Homeostasis Malignant Neoplasms Membrane Metalloproteases Molecular Neoplasm Metastasis Neuroendocrine Cell Neurons Neuropeptides Neurosecretory Systems Normal Cell Pathway interactions Peptide Hydrolases Peptide Signal Sequences Peptide Synthesis Peptides Physiological Postdoctoral Fellow Process Prohormone Convertase Proprotein Convertases Proteins Purpose Range Regulation Research Role Scientist Secretory Vesicles Sorting - Cell Movement Stimulus Students System Tissues Yeasts base genetic analysis human disease insight interest intracellular protein transport membrane biogenesis novel peptide hormone protein structure protein transport secretase secretion process symposium trafficking tumor

项目摘要

DESCRIPTION (provided by applicant): The determination of the molecular and cellular bases of endocrine and neuroendocrine homeostasis is evolving into an exciting but complex field, one that provides insight into a staggering array of human diseases. This is in part due to the integration of powerful disciplines ranging from protein structure and enzymology, to protein trafficking, secretion, and the genetic analysis of signaling peptide synthesis in endocrine homeostasis and the etiology of diseases including diabetes, cancer and Alzheimer's as well as the physiological basis of drug addiction. The identification of the enzymes that catalyze the maturation of hundreds of peptide hormones and neuropeptides was a fundamental step in our understanding of the biosynthesis of signaling peptides and proteins. The study of these enzymes has provided a novel platform to integrate proprotein processing with a variety of cell biological studies, including the regulation of protein traffic and membrane biogenesis, especially Golgi dynamics and sorting, and the formation of dense-core secretory granules in stimulus-coupled endocrine and neuroendocrine cells. Analysis of the Prohormone Convertases and the a-Amidating Enzyme has also provided a foundation to study the role of additional membrane-associated enzymatic systems, particularly the matrix metalloproteases and the secretases that control tissue remodeling and membrane shedding in normal cells, as well as their subversion in promoting tumor metastasis and Alzheimer's disease. The cooperative roles of the secretory pathway protease systems together with the underlying cellular trafficking machinery that contribute to these complex, prevalent diseases provides a potent reason to bring together various disciplines to examine each of these factors as rigorously as possible. The Proprotein Processing, Trafficking and Secretion Gordon Research Conference is unique in bringing together scientists from a variety of disciplines whose common interest is in the synthesis, trafficking, processing, secretion, and function of signaling peptides and proteins. The purpose of this application is to request financial support for young faculty, postdoctoral fellows and graduate students to attend this conference, the only one to integrate and consolidate research from many biological disciplines in order to illuminate the roles of the proprotein convertases, the a- amidating enzyme and other processing enzymes involved in endocrine and CNS homoeostasis and the etiology of human disease. This conference integrates these areas of study into recent advances in the regulation of protein traffic, the control of secretion and exocytosis from yeast to neurons, and novel genetic and biochemical approaches to the study of peptide diversity.

描述（由申请人提供）：内分泌和神经内分泌稳态的分子和细胞碱基的确定正在发展为一个令人兴奋但复杂的领域，该领域可以深入了解各种各样的人类疾病。这部分是由于从蛋白质结构和酶学到蛋白质运输，分泌以及内分泌稳态中信号肽合成的遗传分析以及糖尿病，癌症和阿尔茨海默氏症的生理学基础等疾病的病因的遗传分析的一部分。鉴定催化数百种肽激素和神经肽的成熟的酶是我们对信号肽和蛋白质的生物合成的理解的基本步骤。对这些酶的研究为将普洛蛋白加工与各种细胞生物学研究相结合，包括调节蛋白质流量和膜生物发生，尤其是高尔基体动力学和分类，以及在刺激偶联的内分泌和神经内分泌细胞中形成致密的核心分泌颗粒。对激素转化酶和A增强酶的分析还为研究了其他与膜相关的酶促系统的作用，尤其是基础，尤其是基质金属蛋白酶以及控制组织重塑和膜在正常细胞中脱离组织的分泌酶，以及它们在促进tumor cmoror cmor cmor tumor cmor cmorsis和alzheimerser中的促进。分泌途径蛋白酶系统的合作作用以及有助于这些复杂，普遍的疾病的潜在细胞运输机制提供了有效的理由，可以将各种学科汇总在一起，以尽可能严格地检查这些因素。普罗蛋白的加工，贩运和分泌戈登研究会议在将各种学科的科学家汇集在一起，这些学科的共同兴趣是信号肽和蛋白质的综合，贩运，加工，分泌，分泌以及功能。该申请的目的是为年轻的教师，博士后研究员和研究生提供财政支持，以参加这次会议，这是唯一一项整合和合并许多生物学学科的研究的会议，以照亮与前蛋白转化酶的作用，A-障碍酶和其他加工酶在异源性疾病中涉及人类疾病和cns somestis和Etii cns and Homeosiss和Etii sisi and eTii and eTii sisi and eTii sisi and eTii atsi and ersiei and eTii and eTii and the eTii。该会议将这些研究领域纳入了调节蛋白质流量的最新进展，分泌和胞吐作用从酵母到神经元的控制以及新型的遗传和生化方法，从而研究肽多样性。