The role of reovirus factories in viral RNA regulation.

呼肠孤病毒工厂在病毒 RNA 调节中的作用。

• 批准号: 6955429
• 负责人: CATHY L MILLER
• 金额: $ 16.2万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6955429
• 关键词: RNA; genetics; genome; infection; particle; proteins; role

DESCRIPTION (provided by applicant): The non-fusogenic mammalian orthoreoviruses are members of a family of animal viruses (Reoviridae) that are of considerable importance in human (rotavirus) disease and as oncolytic viruses (reoviruses). Early during reovirus infection small, cytoplasmic, phase-dense inclusions develop which grow in size as infection continues. Viral proteins, as well as partially and fully assembled virions and core particles localize almost exclusively to these structures (termed viral factories) suggesting they are the sites of viral genome replication and assembly. Recently, it has been shown that most viral proteins involved in virus core assembly and genome replication are recruited to the factories through an association with the MNS protein. Moreover, the factories have been found to function as sites for the transcription of viral (+)RNAs, a task that is carried out by core particles, during reovirus infection. This proposal is designed to investigate the role of the reovirus factory in coordinating viral RNA transcription, translation, and replication. The genesis of the viral factory will be investigated to determine at which point in viral infection transcription of viral RNA is located within the protective environment of the factory, and if parental viral core particles direct factory genesis through their association with cellular translation proteins. These experiments will be accomplished by labeling parental cores, then investigating their localization within cells by immunofluorescence, immunoelectron microscopy, and immunoprecipitation studies. Using RNAi technology, the possibility that there are two pools of viral RNA in infected cells, one for replication and one for translation, will be determined. The localization of viral RNA translation and cellular translation factors relative to the localization of viral factories will be examined by immunofluorescence studies to determine if the viral factory, or factory localized proteins direct viral RNA translation. Viral proteins involved in translation will be identified. The role of cis-acting RNA sequences in directing replication within the factory will be investigated by introducing mutations into the viral genome using reverse genetics. These experiments are designed to better understand the role of the viral factory in regulating viral RNA transcription, translation, and replication so that strategies can be developed to interdict and exploit these aspects of reovirus infection.

描述（由申请人提供）：非融合哺乳动物正呼肠孤病毒是动物病毒（呼肠孤病毒科）家族的成员，其在人类（轮状病毒）疾病中具有相当重要的作用并且作为溶瘤病毒（呼肠孤病毒）。在呼肠孤病毒感染早期，会形成小的细胞质相致密包涵体，随着感染的继续，包涵体的尺寸会不断增大。病毒蛋白以及部分和完全组装的病毒粒子和核心颗粒几乎完全定位于这些结构（称为病毒工厂），表明它们是病毒基因组复制和组装的位点。最近，研究表明，大多数参与病毒核心组装和基因组复制的病毒蛋白都是通过与 MNS 蛋白结合而被招募到工厂的。此外，这些工厂被发现充当病毒 (+)RNA 转录的位点，这是呼肠孤病毒感染期间由核心颗粒执行的任务。该提案旨在研究呼肠孤病毒工厂在协调病毒 RNA 转录、翻译和复制中的作用。将研究病毒工厂的起源，以确定病毒感染中病毒RNA转录的哪个点位于工厂的保护环境内，以及亲本病毒核心颗粒是否通过与细胞翻译蛋白的关联来指导工厂的起源。这些实验将通过标记亲本核心来完成，然后通过免疫荧光、免疫电子显微镜和免疫沉淀研究研究它们在细胞内的定位。使用RNAi技术，将确定受感染细胞中存在两种病毒RNA池的可能性，一种用于复制，另一种用于翻译。病毒RNA翻译和细胞翻译因子相对于病毒工厂定位的定位将通过免疫荧光研究进行检查，以确定病毒工厂或工厂定位蛋白是否指导病毒RNA翻译。将鉴定参与翻译的病毒蛋白。通过使用反向遗传学将突变引入病毒基因组，将研究顺式作用 RNA 序列在指导工厂内复制中的作用。这些实验旨在更好地了解病毒工厂在调节病毒 RNA 转录、翻译和复制中的作用，以便制定策略来阻止和利用呼肠孤病毒感染的这些方面。