Next Generation Nano-prism based LSPR chemical sensors.

下一代基于纳米棱镜的 LSPR 化学传感器。

• 批准号: 7056609
• 负责人: JEFFREY N ANKER
• 金额: $ 4.6万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-05 至 2008-12-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056609
• 关键词: amyloid proteins; biomarker; biotechnology; mass spectrometry; monitoring device; nanotechnology; postdoctoral investigator; surface plasmon resonance; technology /technique development

DESCRIPTION (provided by applicant): This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of "amyloid derived diffusable ligands" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases.

描述（由申请人提供）：该项目将开发基于金属纳米棱柱阵列等离子共振的下一代LSPR传感器。 Van Duyne 实验室发明和开发的 LSPR 传感器能够高灵敏度地测量分子浓度和原位表征结合常数。最近，LSPR 传感器测量了阿尔茨海默病和衰老对照组脑脊液中“淀粉样蛋白衍生的可扩散配体”（ADDL）的浓度，这是阿尔茨海默病的一种有前景的生物标志物，也是阿尔茨海默病的可能原因。初步结果显示阿尔茨海默氏症患者的水平较高，进一步的测试正在进行中。该项目通过以下方式增强了现有技术：1) 优化纳米棱柱形状和化学性质，并通过改变 pH、温度和离子强度来调节结合。 2）将纳米棱柱沉积到柔性且可拉伸的表面上以调节纳米棱柱间距和电磁场耦合。 3) 将 LSPR 底物与质谱 (MS) 仪器连接，以进行免费结合研究和 MS 鉴定。这些传感器有望用于 ADDL 化学研究以及阿尔茨海默病的诊断和治疗的持续评估。该技术也可用于其他生物标志物和疾病。