NMR Cryoprobe for Structural Biology Research

用于结构生物学研究的 NMR 冷冻探针

基本信息

批准号：
6878284
负责人：
Ishwar Radhakrishnan
金额：
$ 27.16万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-01-15 至 2006-01-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the acquisition of a cryoprobe for substantially increasing the sensitivity of the high-resolution 600 MHz NMR instrument in the WCAS Structural Biology NMR Facility at Northwestern University. With previous NIH and NSF support, Northwestern structural biologists established a resource for macromolecular NMR that has yielded significant results. The 600 MHz spectrometer, which is the only NMR instrument in the facility, is used heavily and continuously. The two- to three-fold increase in sensitivity afforded by cryoprobes over conventional third generation probes translates into four- to nine-fold reduction in acquisition times, thereby significantly enhancing throughput. The acquisition of a cryoprobe will thus more effectively meet the growing scientific needs of as many as six research groups that currently use the instrument. It also represents a cost-effective solution since the acquisition costs of a new 600 MHz instrument is significantly higher than that of a cryoprobe. An added benefit of the increased sensitivity of the cryoprobe is the lowered sample concentration requirements. Thus, studies of samples that are expensive to produce, prone to aggregate at high concentrations, or are otherwise chemically unstable are readily facilitated, thus opening up the possibility of studying research problems previously inaccessible by this technique. The cryoprobe we propose to acquire will have all the capabilities of third generation probes for macromolecular NMR including optimized direct detection of 1H frequencies, simultaneous irradiation at 1H, 2H, 13C, and 15N frequencies, and actively shielded z-axis gradient. Cryogenic cooling of the probe requires a closed-cycle cryogenic system comprising a helium refrigerator, a temperature controller, a helium compressor, and a turbo vacuum pump as well as a water chiller unit as essential accessories. The cryogenic system and the water chiller are fully compatible with and can be readily accommodated in the current layout of the NMR facility with only minor modifications to the facility. Approval of the site plan has been already obtained from the manufacturer. The cryoprobe along with the closed-cycle cryogenic system and water chiller as accessories will thus reestablish the 600 MHz instrument in the WCAS facility as a state-of-the-art resource for macromolecular NMR.

描述（由申请人提供）：该提案请求资金购买冷冻探针，以大幅提高西北大学 WCAS 结构生物学 NMR 设施中高分辨率 600 MHz NMR 仪器的灵敏度。在 NIH 和 NSF 之前的支持下，西北大学结构生物学家建立了大分子 NMR 资源，并取得了重大成果。 600 MHz 光谱仪是该设施中唯一的 NMR 仪器，持续大量使用。与传统的第三代探针相比，冷冻探针的灵敏度提高了两到三倍，这意味着采集时间减少了四到九倍，从而显着提高了通量。因此，购买冷冻探针将更有效地满足目前使用该仪器的多达六个研究小组不断增长的科学需求。它还代表了一种经济高效的解决方案，因为新的 600 MHz 仪器的采购成本明显高于冷冻探针。冷冻探针灵敏度提高的另一个好处是降低了样品浓度要求。因此，对生产成本昂贵、在高浓度下容易聚集或化学不稳定的样品的研究很容易得到促进，从而为研究以前通过该技术无法解决的研究问题提供了可能性。我们建议购买的冷冻探针将具有第三代大分子 NMR 探针的所有功能，包括优化的 1H 频率直接检测、1H、2H、13C 和 15N 频率同时照射以及主动屏蔽 z 轴梯度。探头的低温冷却需要闭式循环低温系统，包括氦制冷机、温度控制器、氦压缩机、涡轮真空泵以及冷水机组作为重要配件。低温系统和冷水机与 NMR 设施的当前布局完全兼容，并且只需对设施进行少量修改即可轻松适应。场地规划已获得制造商的批准。因此，冷冻探针连同闭式循环低温系统和冷水机作为配件，将在 WCAS 设施中重建 600 MHz 仪器，作为大分子 NMR 的最先进资源。

项目成果

期刊论文数量（27）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Solution NMR Studies of an Alternative Mode of Sin3 Engagement by the Sds3 Subunit in the Histone Deacetylase-Associated Sin3L/Rpd3L Corepressor Complex.

组蛋白脱乙酰酶相关 Sin3L/Rpd3L 辅阻遏物复合体中 Sds3 亚基与 Sin3 接合的替代模式的溶液 NMR 研究。

DOI：
发表时间：
2015-12-04
期刊：
影响因子：
5.6
作者：
Clark, Michael David;Zhang, Yongbo;Radhakrishnan, Ishwar
通讯作者：
Radhakrishnan, Ishwar

Light-Driven Expansion of Spiropyran Hydrogels.

螺吡喃水凝胶的光驱动膨胀。

DOI：
10.1021/jacs.0c02201
发表时间：
2020-04-24
期刊：
Journal of the American Chemical Society
影响因子：
15
作者：
Chuang Li;Aysenur Iscen;Liam C. Palmer;G. Schatz;S. Stupp
通讯作者：
S. Stupp

Fast and programmable locomotion of hydrogel-metal hybrids under light and magnetic fields.

水凝胶-金属混合物在光和磁场下的快速、可编程运动。

DOI：
发表时间：
2020
期刊：
影响因子：
25
作者：
Li, Chuang;Lau, Garrett C;Yuan, Hang;Aggarwal, Aaveg;Dominguez, Victor Lopez;Liu, Shuangping;Sai, Hiroaki;Palmer, Liam C;Sather, Nicholas A;Pearson, Tyler J;Freedman, Danna E;Amiri, Pedram Khalili;de la Cruz, Monica Olvera;Stupp, Samuel I
通讯作者：
Stupp, Samuel I

Syntheses of three-dimensional catenanes under kinetic control.

动力学控制下三维索烷的合成。

DOI：
发表时间：
2022-03-22
期刊：
影响因子：
11.1
作者：
Wu, Yong;Guo, Qing;Qiu, Yunyan;Weber, Jacob A;Young, Ryan M;Bancroft, Laura;Jiao, Yang;Chen, Hongliang;Song, Bo;Liu, Wenqi;Feng, Yuanning;Zhao, Xingang;Li, Xuesong;Zhang, Long;Chen, Xiao;Li, Hao;Wasielewski, Michael R;Stoddart, J
通讯作者：
Stoddart, J

Quantum Dot-Catalyzed Photoreductive Removal of Sulfonyl-Based Protecting Groups.