600 MHz NMR Spectrometer for Shared Use

共享使用的 600 MHz 核磁共振波谱仪

• 批准号: 6878258
• 负责人: R Blake Hill
• 金额: $ 50万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6878258
• 关键词: biochemistry; biomedical equipment purchase; biophysics; nuclear magnetic resonance spectroscopy

DESCRIPTION (provided by applicant): A high-field NMR spectrometer, operating at a 1H resonance frequency of 600 MHz and fully equipped for biomolecular applications, is requested to meet the needs of a broad group of biophysical and biochemical investigators at the Homewood campus at Johns Hopkins University. The requested instrumentation will be sited in a newly completed NMR Center, constructed in accordance with the University's long term goal of providing state-of-the-art capabilities for fundamental investigations of biomolecular structure and dynamics. This instrumentation will significantly benefit the research programs of seven faculties from the Departments of Biology, Biophysics, and Chemistry, of which six are NIH-funded. A range of projects of biological, biophysical, and chemical significance are described: (1) Structure and dynamics studies of proteins involved in mitochondrial division. (2) NMR studies of macromolecular structure and dynamics and its relationship to biological function. (3) Long range effects of amino acid polymorphisms and mutations causing drug-resistant in the HIV-1 protease. (4) Structural and energetic principles of membrane proteins. (5) Correlation between structure and energy in proteins. (6) Structure determination of the DNA-binding domain of AraC and dynamics in the presence and absence of its cognate DNA. (7) Thermodynamic analysis of backbone and side chain dynamics of DNA binding proteins in the presence and absence of DNA.

描述（由申请人提供）：需要一台高场核磁共振波谱仪，工作频率为 600 MHz 的 1H 共振频率，并且完全适合生物分子应用，以满足霍姆伍德校区广大生物物理和生化研究人员的需求。约翰·霍普金斯大学。所需的仪器将安置在新建成的核磁共振中心，该中心是根据大学的长期目标而建造的，即为生物分子结构和动力学的基础研究提供最先进的能力。该仪器将大大有利于生物学、生物物理学和化学系七个院系的研究项目，其中六个院系由 NIH 资助。描述了一系列具有生物学、生物物理和化学意义的项目：（1）参与线粒体分裂的蛋白质的结构和动力学研究。 (2)大分子结构和动力学及其与生物功能关系的NMR研究。 (3)氨基酸多态性和突变导致HIV-1蛋白酶产生耐药性的长程影响。 (4)膜蛋白的结构和能量原理。 (5)蛋白质结构与能量的相关性。 (6) AraC DNA 结合结构域的结构测定以及其同源 DNA 存在和不存在时的动力学。 (7)在存在和不存在DNA的情况下，对DNA结合蛋白的主链和侧链动力学进行热力学分析。