Acquisition of a MALDI-TOF High Resolution Mass Spec

获取 MALDI-TOF 高分辨率质谱仪

• 批准号: 7037944
• 负责人: CLIFF SOLL
• 金额: $ 40.89万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-29 至 2007-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7037944
• 关键词: biomedical equipment purchase; biophysics; mass spectrometry; matrix assisted laser desorption ionization

DESCRIPTION (provided by applicant): The City University of New York (CUNY) is the nation's largest urban university. There are approximately 120 faculty in chemistry and biochemsitry. CUNY graduates -8% of the minority Ph.D.'s in these fields yearly. The Hunter College/CUNY Mass Spectrometry Facility currently collaborates with 25 laboratories throughout CUNY, and 10 laboratories at other educational institutions including 3 medical schools. There are no high resolution mass spectrometers in the CUNY system causing investigators to send samples outside, many times waiting considerable time in order to get their results. In addition, it is difficult to get outside laboratories to conduct non routine experiments. In order to fill this void, this proposal is requesting funding for a state-of-the-art HPLC Time-of-Flight (TOF) high resolution mass spectrometer system with various ionization sources (MALDI/ESI/APCI). The proposed system will serve a broad spectrum of NIH investigators at Hunter College and the City University of New York (CUNY) system (10 listed in this proposal). The instrumentation will provide empirical formula confirmations for small bioactive molecules and molecular weight confirmations of intact proteins and oligonucleotides. Analysis of protein digests in order to identify proteins and post translational modifications will also be performed. The instrumentation will be located in the CUNY Mass Spectrometry Facility at Hunter College and will be overseen by the facility director, a full-time Ph.D. chemist, who has successfully managed the facility for the last 10 years. Maintenance and supply costs beyond the warranty period will be paid for by user fees, a mechanism that has been successfully used in this facility for the last 7 years. The mass Spectrometry instrumentation requested will provide key scientific data to researchers in the chemical, biochemical and biological sciences. This data will be used to help elucidate chemical structure and study the molecular basis for disease.

描述（由申请人提供）：纽约市立大学 (CUNY) 是美国最大的城市大学。化学和生物化学专业约有 120 名教师。纽约市立大学每年从这些领域的少数族裔博士毕业生中毕业 -8%。亨特学院/纽约市立大学质谱设施目前与纽约市立大学的 25 个实验室以及其他教育机构（包括 3 所医学院）的 10 个实验室合作。纽约市立大学系统中没有高分辨率质谱仪，导致研究人员将样本送到外面，很多时候要等待相当长的时间才能得到结果。此外，很难让外部实验室进行非常规实验。为了填补这一空白，该提案请求资金用于配备各种电离源（MALDI/ESI/APCI）的最先进的 HPLC 飞行时间 (TOF) 高分辨率质谱仪系统。拟议的系统将为亨特学院和纽约市立大学 (CUNY) 系统（本提案中列出的 10 名）的广泛 NIH 研究人员提供服务。该仪器将为生物活性小分子提供经验公式确认，并为完整蛋白质和寡核苷酸提供分子量确认。还将进行蛋白质消化分析，以鉴定蛋白质和翻译后修饰。该仪器将位于亨特学院的纽约市立大学质谱设施中，并由设施主任、全职博士生负责监督。化学家，过去 10 年成功管理该设施。保修期外的维护和供应费用将由使用费支付，这一机制在过去七年中已在该设施中成功使用。所需的质谱仪器将为化学、生物化学和生物科学的研究人员提供关键的科学数据。这些数据将用于帮助阐明化学结构并研究疾病的分子基础。

项目成果

Inactivation of tesA reduces cell wall lipid production and increases drug susceptibility in mycobacteria.

tesA 失活会减少分枝杆菌细胞壁脂质的产生并增加药物敏感性。

• 发表时间: 2011-07-15
• 作者: Chavadi, Sivagami Sundaram;Edupuganti, Uthamaphani R;Vergnolle, Olivia;Fatima, Itrat;Singh, Shaneen M;Soll, Clifford E;Quadri, Luis E N
• 通讯作者: Quadri, Luis E N

Isolation and identification of oligomers from partial degradation of lime fruit cutin.

酸橙果角质部分降解低聚物的分离和鉴定。

• 发表时间: 2008-11-12
• 影响因子: 6.1
• 作者: Tian, Shiying;Fang, Xiuhua;Wang, Weimin;Yu, Bingwu;Cheng, Xiaofang;Qiu, Feng;Mort, Andrew J;Stark, Ruth E
• 通讯作者: Stark, Ruth E