Acquisition of an MALDI TOF-TOF Mass Spectrometer

购买 MALDI TOF-TOF 质谱仪

基本信息

批准号：
6803737
负责人：
ELIZABETH A. KOMIVES
金额：
$ 75.81万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-06-01 至 2006-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6803737
关键词：
biomedical equipment purchase matrix assisted laser desorption ionization

项目摘要

DESCRIPTION (provided by applicant): The Mass Spectrometry Facility at the University of California San Diego wishes to acquire an Applied Biosystems 4700 Matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF-TOF) mass spectrometer. This mass spectrometer has novel patented tandem sources in which collision-induced dissociation occurs from the high-energy (1-2 keV) singly charged parent ions generated by MALDI. This technology is critical for determining the protein functional landscape, the next step after completion of the human genome project. The genome sequence revealed many fewer proteins than expected, suggesting that proteins have multiple states with differing functions. Protein function can be altered by participation in multiprotein complexes, post-translational modification state, and these modifications can result in different conformational states. The MALDI-TOF-TOF instrument will be used to approach all three of these questions. First, we will identify proteins in complexes in a robust and comprehensive way, even when the genome sequences of the organism from which the complex was isolated are not available. Second, we will identify the post-translational modifications on each of the proteins in the complex, beginning with glycosylation and phosphorylation. Third, we will identify the individual amino acids on surfaces of the proteins that interact in the complex, and understand the conformational changes that accompany binding. Professors H. Zhou, J. Dixon, S. Taylor, R. Tsien, B. Tebo, and P. van der Geer are engaged in development of proteomics experiments to identify proteins in cellular complexes. Professors Zhou and P. Taylor are studying phosphorylation, glycosylation, and modification of proteins by environmental agents. Professor E. Komives pioneered the use of MALDI amide H/2H exchange for identification of protein interfaces and conformational changes that accompany protein-protein interactions. For this project, the MALDI-TOF-TOF will enable measurements of amide deuteration down to the single amino acid level.

描述（由申请人提供）：加州大学圣地亚哥分校的质谱设施希望购买一台 Applied Biosystems 4700 矩阵辅助激光解吸电离 (MALDI) 串联飞行时间 (TOF-TOF) 质谱仪。该质谱仪具有新颖的专利串联源，其中由 MALDI 生成的高能 (1-2 keV) 单电荷母离子发生碰撞诱导解离。这项技术对于确定蛋白质功能景观至关重要，这是人类基因组计划完成后的下一步。基因组序列显示的蛋白质比预期少得多，这表明蛋白质具有具有不同功能的多种状态。蛋白质功能可以通过参与多蛋白复合物、翻译后修饰状态而改变，并且这些修饰可以导致不同的构象状态。 MALDI-TOF-TOF 仪器将用于解决所有这三个问题。首先，我们将以稳健且全面的方式鉴定复合物中的蛋白质，即使无法获得分离复合物的生物体的基因组序列。其次，我们将确定复合物中每种蛋白质的翻译后修饰，从糖基化和磷酸化开始。第三，我们将识别在复合物中相互作用的蛋白质表面上的各个氨基酸，并了解伴随结合的构象变化。 H. Zhou、J. Dixon、S. Taylor、R. Tsien、B. Tebo 和 P. van der Geer 教授致力于蛋白质组学实验的开发，以鉴定细胞复合物中的蛋白质。 Zhou 教授和 P. Taylor 正在研究环境因素对蛋白质的磷酸化、糖基化和修饰。 E. Komives 教授率先使用 MALDI 酰胺 H/2H 交换来识别蛋白质界面和伴随蛋白质-蛋白质相互作用的构象变化。对于该项目，MALDI-TOF-TOF 将能够测量低至单个氨基酸水平的酰胺氘化。