Role of Cell Death Pathway in C. elegans Innate Immunity

细胞死亡途径在秀丽隐杆线虫先天免疫中的作用

• 批准号: 7384403
• 负责人: NATHAN C GADDIS
• 金额: $ 5.67万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7384403
• 关键词: Affect; Apoptosis; Apoptotic; Area; Biological Models; Caenorhabditis elegans; Caspase; Cell Death; Dissection; Fellowship; Generations; Genes; Genetic; Genome; Goals; Immune response; Individual; Localized; Location; Mammals; Measures; Molecular Profiling; Mucins; Names; Natural Immunity; Nematoda; Organism; Pathway interactions; Predisposition; RNA Interference; Regulation; Relative (related person); Research; Role; Salmonella enterica; Time; Work; defense response; insight; pathogen; tool

The nematode Caenorhabditis elegans has recently emerged as a powerful model system for studying innate immunity. The short generation time of C. elegans, in combination with the abundance of well-defined genetic tools available for its study, makes it an ideal organism for the rapid dissection of innate immune response pathways. Previous studies have indicated that components of the C. elegans CED-3 programmed cell death pathway are required for defense of the worm against certain pathogens. The goal of the research described in this proposal is to elucidate the exact mechanism by which the CED-3 pathway provides protection. In particular, the aims are to: 1) Identify the location of defense-related CED-3-pathway activity within C. elegans, 2) Characterize the role of CED-3-regulated mucins in the defense response and 3) Define the genetic pathway leading from interaction with pathogen to expression of CED-3-regulated mucins. Importantly, because many innate immunity pathways appear to be highly conserved evolutionary, these studies could potentially provide insights not only into the C. elegans defense response, but also into unknown aspects of mammalian innate immunity.

线虫秀丽隐杆线虫最近已成为一种强大的模型系统，用于研究 先天免疫。秀丽隐杆线虫的短期，结合了大量明确的时间 可用于研究的遗传工具使其成为快速解剖先天免疫的理想生物 响应途径。先前的研究表明，秀丽隐杆线虫CED-3的成分 需要程序性细胞死亡通路来防御某些病原体。目标 该提案中描述的研究是阐明CED-3途径的确切机制 提供保护。特别是，目的是：1）确定与防御相关的CED-3-Pathway的位置 秀丽隐杆线虫内的活动，2）表征CED-3调节的粘蛋白在防御反应中的作用 3）定义从与病原体相互作用到CED-3调节的表达的遗传途径 粘蛋白。重要的是，由于许多先天免疫途径似乎是高度保守的进化，所以 这些研究可能不仅可以为秀丽隐杆线虫的防御反应提供见解，还可以提供 哺乳动物先天免疫的未知方面。