Fracture healing assessment by real-time and noninvasive Raman spectorscopy

通过实时、无创拉曼光谱评估骨折愈合情况

• 批准号: 7486400
• 负责人: Jacqueline H Cole
• 金额: $ 4.68万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7486400
• 关键词: Age; Age-Months; Architecture; Biochemical; Biological Products; Bone Growth; Bone Matrix; Bone Regeneration; Bone Tissue; Bone callus; Clinical; Contralateral; Decompression Sickness; Dentin; Detection; Development; Diaphyses; Disease; Environment; Epiphysial cartilage; Fracture; Fracture Healing; Future; Goals; Growth; Harvest; Healed; Health; Hour; Image; Image Analysis; Impaired wound healing; In Vitro; Injury; Invertebrates; Kinetics; Laboratories; Measurement; Mechanics; Metabolic; Metabolic Diseases; Minerals; Modeling; Monitor; Mus; Normal Range; Operative Surgical Procedures; Phase; Physiologic Ossification; Physiologic calcification; Process; Property; Proteins; Quality of life; Raman Spectrum Analysis; Shapes; Site; Skeletal Development; Skeletal system; Skeleton; Staging; System; Techniques; Testing; Thinking; Tibial Fractures; Time; Tissues; Week; Wild Type Mouse; X-Ray Computed Tomography; age group; biomineralization; bone; calcium phosphate; carboapatite; day; fetal; healing; in vivo; insight; male; middle age; mineralization; novel; octacalcium phosphate; repaired; research study; tibia; tool; young adult

DESCRIPTION (provided by applicant): Bone fractures disrupt essential metabolic and mechanical functions of the skeleton and thus threaten health and quality of life. Fracture healing attempts to restore the shape, function, and material properties of bone. Biomineralization is thought to proceed through a sequence of transient phases before reaching a stable form, a progression that has been observed in vitro for invertebrates and dentin and recently was discovered in vivo for fetal murine tissue by the Morris group. However, the biochemical mechanisms behind bone tissue mineralization, particularly during fracture healing, are still not well understood. While fracture callus mineralization is thought to be similar to growth plate mineralization, the occurrence of transient mineral phases during fracture repair have not been examined in real time. The hypotheses for this study are that (1) fracture healing includes a mineral transformation from a disordered phase through a transient phase to a final stable phase, and this mechanism is similar regardless of skeletal age and stage of development; and (2) biochemical composition of the fracture callus can be monitored noninvasively throughout the healing process, and these measurements are predictive of callus material quality. To test the hypotheses, mineralization kinetics will be followed for the fracture callus at the femoral middiaphysis of male wild type mice. Raman spectroscopy will examine the kinetics over a 24-hour period in the early stages of fracture repair for mice of different ages. Next, bone matrix and mineral properties will be studied in the fracture callus throughout healing in skeletally mature male wild type mice. The biochemical properties observed by noninvasive Raman spectroscopy will be correlated with geometric and mechanical properties to assess the ability of this technique to characterize the fracture callus integrity. The proposed study will provide unique information about the progression of tissue mineralization during fracture healing and will assess the ability of a noninvasive Raman spectroscopic system to monitor the healing process. Overall, the goals of these experiments are to gain insights into the mechanisms of fracture healing mineralization and to provide a clinical imaging and analysis tool to assess the fracture site noninvasively, regardless of age. This type of information could be invaluable in the detection and correction of abnormal healing and thus may be useful for developing strategies to promote successful bone repair.

描述（由申请人提供）：骨折破坏骨骼的基本代谢和机械功能，从而威胁到健康和生活质量。断裂愈合试图恢复骨骼的形状，功能和材料特性。人们认为生物矿化是在达到稳定形式之前通过一系列瞬态相处进行的，这是无脊椎动物和牙本质在体外观察到的进展，最近在体内发现了莫里斯组的胎儿组织。然而，骨组织矿化背后的生化机制，尤其是在裂缝愈合过程中，仍然尚不清楚。虽然裂缝愈伤组织矿化被认为与生长板矿化相似，但尚未实时检查裂缝修复过程中瞬时矿物相的发生。这项研究的假设是（1）断裂愈合包括从无序相到瞬态到最终稳定相的矿物质转化，而无论骨骼年龄和发育阶段如何，这种机制都相似。 （2）在整个愈合过程中，可以无创监测裂缝愈伤组织的生化组成，这些测量值可预测愈伤组织材料质量。 为了检验假设，将在男性野生型小鼠的股骨中部骨中骨折的裂缝进行矿化动力学。拉曼光谱学将在24小时内的动力学在不同年龄的小鼠的骨折修复的早期阶段检查动力学。接下来，将在整个骨骼成熟的雄性野生型小鼠的愈合过程中研究骨基质和矿物质特性。通过非侵入性拉曼光谱观察到的生化特性将与几何和机械性能相关，以评估该技术表征断裂愈伤组织完整性的能力。拟议的研究将提供有关裂缝愈合过程中组织矿化进展的独特信息，并将评估非侵入性拉曼光谱系统监测愈合过程的能力。总体而言，这些实验的目标是了解骨折愈合矿化机制，并提供临床成像和分析工具，以无创地评估骨折部位，无论年龄如何。这种信息在异常愈合的检测和纠正中可能是无价的，因此对于制定促进成功骨修复的策略可能很有用。