Imaging Core

成像核心

• 批准号: 7438489
• 负责人: Steven Mark Larson
• 金额: $ 32万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-05 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7438489
• 关键词: Animals; Autoradiography; Biological Assay; Bioluminescence; Cell Proliferation; Development; Fire - disasters; Fluorescence; Genetic; Glycolysis; Heat-Shock Proteins 70; Human; Hypoxia; Hypoxia Inducible Factor; Image; Imaging Techniques; Imaging technology; Individual; Invasive; Longitudinal Studies; Memorial Sloan-Kettering Cancer Center; Metabolic; Modality; Monitor; Numbers; Optics; Patients; Positron-Emission Tomography; Radioisotopes; Radionuclide Imaging; Reporter; Reporter Genes; Research Project Grants; Response Elements; System; TP53 gene; Techniques; Translating; base; in vivo; malignant breast neoplasm; molecular imaging; mouse model; neoplastic cell; non-invasive system; single photon emission computed tomography; trafficking; tumor

The theme of the Imaging Core is molecular imaging of mouse models of human breast cancer. The Core provides individual projects with non-invasive, quantitative imaging-based capabilities for metabolic and genetic characterization of tumors and their microenvironment, including in vivo trafficking of tumor cells. This will be accomplished by monitoring of "directly-targeting" probes and of the expression of single and multi-modality reporter genes using optical (bioluminescence and fluorescence), radionuclide (PET, SPECT, and autoradiography), MRI/MRS, CT, and US) imaging. These quantitative, non-invasive imaging techniques are well-established at MSKCC for both smallanimal and patient imaging studies. They are quantitative and non-invasive and thus readily adaptable to longitudinal studies. The Imaging Core can readily provide [18F]-FDG, [18F]-FLT, [18F]-ACBC , and [I8F]-FMISO or [124I]-IAZG microPET to all RPs for non-invasive assessment of tumor glycolysis, cell proliferation, amino transport, and hypoxia, respectively, in mouse models of breast cancer. Further, a number of inducible reporter systems for non-invasive in vivo imaging in small animals have been developed. These reporter systems are all multi-modality reporters and include the capability for fluorescence, bioluminescence and radionuclide imaging. In addition to p53 and DFHR, the list of inducible reporters includes NFAT, FIRE (hypoxia response element - hypoxia inducible factor), E2F, Forkhead factor (FOXO), heat shock protein 70 (HSP70) and TGFp. A general two-step strategy is generally pursued in probe development: first, to establish and validate imaging and non-invasive assay techniques in experimental animals, and second, to translate where appropriate selected aspects of our imaging technology to patient studies within the context of the Research Projects (RPs) proposed in this Application. The Imaging Core will actively and directly support all five RPs, led by Harold Varmus, Joan Massague, Neil Rosen, Maria Jasin, and Robert Benezra, respectively.

成像核心的主题是人类乳腺癌小鼠模型的分子成像。 核心为单个项目提供了非侵入性，基于定量成像的功能 肿瘤及其微环境的代谢和遗传表征，包括体内 肿瘤细胞的运输。这将通过监视“直接靶向”探针和 使用光学发光和多模式报告基因的表达（生物发光和 荧光），放射性核素（PET，SPECT和放射自显影），MRI/MRS，CT和US成像。 这些定量的，非侵入性成像技术在MSKCC上都是良好的 和患者成像研究。它们是定量的，无创的，因此很容易适应 纵向研究。成像核可以很容易地提供[18f] -fdg，[18f] -flt，[18f] -acbc和 [i8f] -fmiso或[124i] -iazg micropet到所有RPS，用于无创评估肿瘤糖酵解，细胞 在乳腺癌小鼠模型中，分别扩散，氨基转运和缺氧。此外， 小动物中非侵入性体内成像的诱导型记者系统数量已经 发达。这些记者系统都是多模式的记者，包括 荧光，生物发光和放射性核素成像。除了p53和dfhr， 诱导记者包括NFAT，火灾（缺氧反应元件 - 缺氧诱导因子），E2F， 叉头因子（FOXO），热激蛋白70（HSP70）和TGFP。一般的两步策略是 通常在探针开发中追求：首先，建立和验证成像和非侵入性测定 实验动物的技术，其次是在我们的适当选择的地方翻译我们的技术 在此提出的研究项目（RPS）的背景下对患者研究进行成像技术 应用。成像核心将积极并直接支持所有五个RP，由Harold Varmus领导， Joan Massague，Neil Rosen，Maria Jasin和Robert Benezra分别。