CORE PROJECT

核心项目

• 批准号: 6933053
• 负责人: Paul A Fuchs
• 金额: $ 62.48万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-23 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933053
• 关键词: biomedical facility; neurosciences

DESCRIPTION (provided by applicant): This is an application from the Center for Hearing and Balance at Johns Hopkins University School of Medicine for a Research Core Grant from the NIDCD. The Center includes eleven investigators drawn from the departments of Otolaryngology- Head and Neck Surgery, and Biomedical Engineering. These laboratories provide a Research Base of nine R01 awards with scientific aims ranging from the molecular biophysics of hair cells to behavioral assays of hearing and balance. Three Research Cores are proposed that will facilitate ongoing research and stimulate new initiatives at the interface between existing programs. The Molecular Biology Core will provide training for and access to molecular genetic research tools including quantitative polymerase chain reaction and generation and assessment of molecular probes for in situ hybridization and immunohistology. A 'real-time' fluorescent monitor thermal cycler will be available through this Core. The Histology Core will provide technical assistance and material support for light and electron microscopic studies. Users will receive guidance in tissue preparation, sectioning, and visualization techniques. Fluorescence microscopy and a digital imaging station will be available through the Core. The Engineering Core will develop and distribute advanced data acquisition and signal analysis software, and provide general hardware and software support for Center laboratories. An Administrative unit will oversee operations throughout the Cores, design and maintain a central Website, provide clerical and personnel support and prepare reports.

描述（由申请人提供）：这是约翰·霍普金斯大学医学院听力与平衡中心向 NIDCD 提供的研究核心拨款的申请。该中心包括来自耳鼻喉头颈外科和生物医学工程部门的 11 名研究人员。这些实验室提供了九个 R01 奖项的研究基地，其科学目标涵盖从毛细胞的分子生物物理学到听力和平衡的行为分析。提出了三个研究核心，这将促进正在进行的研究，并在现有计划之间的接口处激发新的举措。 分子生物学核心将为分子遗传学研究工具提供培训和使用，包括定量聚合酶链反应以及原位杂交和免疫组织学分子探针的生成和评估。通过该核心将提供“实时”荧光监测热循环仪。 组织学核心将为光和电子显微镜研究提供技术援助和物质支持。用户将收到组织准备、切片和可视化技术方面的指导。荧光显微镜和数字成像站将通过核心提供。 工程核心将开发和分发先进的数据采集和信号分析软件，并为中心实验室提供通用硬件和软件支持。 行政部门将监督整个核心的运营，设计和维护中央网站，提供文书和人员支持并准备报告。