Acquisition of an Automated Tissue Processor for the ASU Shared Imaging Core Facility

为 ASU 共享成像核心设施采购自动组织处理机

• 批准号: 10737175
• 负责人: DAVID A BRAFMAN
• 金额: $ 5.97万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10737175
• 关键词: Adverse effects; Alcohols; Area; Arizona; Biocompatible Materials; Biomanufacturing; Biomedical Research; Biophysics; Cell physiology; Cells; Cellular biology; Communicable Diseases; Communities; Computers and Advanced Instrumentation; Core Facility; Data Management Resources; Dehydration; Disease model; Educational process of instructing; Engineering; Ensure; Equipment; Equipment and supply inventories; Extramural Activities; Faculty; Fee-for-Service Plans; Fixatives; Fluorescent in Situ Hybridization; Funding; Future; Genome engineering; Histologic; Histology; Hour; Human Resources; Image; Immunology; Institution; Interdisciplinary Study; Investigation; Laboratories; Laboratory Research; Location; Manuals; Methodology; Microscopic; Microscopy; Modernization; Molecular Biology; Nanotechnology; Nerve Degeneration; Neurosciences; Organ; Paraffin; Patients; Phylogenetic Analysis; Postdoctoral Fellow; Preparation; Procedures; Process; Proteins; RNA; Regenerative Medicine; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Sampling; Slice; Speed; Structure; System; Technology; Thinness; Time; Tissue Engineering; Tissues; Training; Translational Research; United States; United States National Institutes of Health; Universities; Veins; Water; Waxes; absorption; animal facility; cancer cell; catalyst; drug discovery; equipment acquisition; experimental study; graduate student; histological specimens; improved; instrument; instrumentation; light microscopy; operation; recruit; research facility; stem cells; synthetic biology; tissue processing; tissue stem cells; tool; translational medicine

PROJECT SUMMARY ABSTRACT Since 2008, the Advanced Light Microscopy Facility (ALMF) has been serving the research needs of a diverse set of research groups. Currently, the ASU ALMF regularly serves the research needs of over 40 research laboratories across 6 academic units. Critically, this facility serves as the only shared core laboratory that provides microscopy and histology capabilities to the researches on the ASU Tempe campus. The current histology equipment located in the core was purchased in 2010 and only provides for labor-intensive manual processing of samples. Thus, this equipment has outgrown both the histology capacity and technology needs required by new faculty as well as current researchers who have enhanced needs. To that end, this proposal is requested for the acquisition of an automated tissue processor that will allow for in depth histological analysis. Overall, this integrated, semi-automated histology processor will be used by researchers to characterize a diverse set of cells tissues from stem cells, primary tissue, cancer cells, and patient samples. As such, this advanced instrumentation will significantly enhance the broad research capabilities at ASU in the areas of engineering (including biophysics, nanotechnology, biomaterials), synthetic biology (including biomanufacturing, genome engineering, biofuels), regenerative medicine (including tissue engineering, disease modeling, and drug discovery), cellular and molecular biology (including cell physiology, neuroscience, phylogenetics), and translational medicine (including neurodegeneration, infectious disease, immunology). Moreover, acquisition of this advanced instrumentation will provide collaborative opportunities for ASU investigators, aid in recruitment of new faculty, and serve as a catalyst for future extramural funding. In addition to expanding and enhancing interdisciplinary research at ASU, the acquisition of cutting-edge histological technology will augment current training and teaching activities throughout the campus. Numerous postdoctoral fellows and graduate students will be trained to perform experiments through faculty-driven research projects. Overall, the requested instrument will provide a critical resource to all biomedical researchers at one of the largest public universities in the United States.

项目概要摘要 自 2008 年以来，先进光学显微镜设施 (ALMF) 一直致力于满足不同领域的研究需求 一组研究小组。目前，ASU ALMF 定期满足 40 多个研究项目的研究需求 跨 6 个学术单位的实验室。至关重要的是，该设施是唯一的共享核心实验室 为亚利桑那州立大学坦佩校区的研究提供显微镜和组织学能力。目前的 位于核心的组织学设备于 2010 年购买，仅提供劳动密集型手册 样品的处理。因此，该设备已经超出了组织学能力和技术需求 新教师以及现有研究人员的需求有所增加。为此，本提案是 请求购买自动组织处理机，以便进行深入的组织学分析。 总体而言，研究人员将使用这种集成的半自动组织学处理器来表征 来自干细胞、原代组织、癌细胞和患者样本的不同细胞组织。因此，这 先进的仪器将显着增强亚利桑那州立大学在以下领域的广泛研究能力 工程学（包括生物物理学、纳米技术、生物材料）、合成生物学（包括生物制造、 基因组工程、生物燃料）、再生医学（包括组织工程、疾病建模和药物 发现）、细胞和分子生物学（包括细胞生理学、神经科学、系统发育学），以及 转化医学（包括神经退行性疾病、传染病、免疫学）。此外，收购 这种先进的仪器将为亚利桑那州立大学的研究人员提供合作机会，帮助招募 新教师，并作为未来校外资助的催化剂。除了扩大和加强 亚利桑那州立大学的跨学科研究，尖端组织学技术的获取将增强当前的 整个校园的培训和教学活动。多名博士后和研究生 将接受培训以通过教师驱动的研究项目进行实验。总体而言，所要求的仪器 将为美国最大的公立大学之一的所有生物医学研究人员提供重要资源 国家。