CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 7055788
• 负责人: George B Witman
• 金额: $ 5.22万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-11 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7055788
• 关键词: bioimaging /biomedical imaging; biomedical facility; diabetes mellitus; electron microscopy; scanning electron microscopy; tissue /cell preparation; transmission electron microscopy

The goal of the DERC Electron Microscopy (EM) Core Facility is to provide complete, first-rate EM service to DERC researchers at UMMS. The Facility is centrally located in a newly renovated suite in the basement of the Basic and Clinical Sciences Building. It is directed by Drs. George Witman and Roger Craig, and managed on a day-to-day basis by Dr. Gregory Hendricks, a full-time professional electron microscopist with extensive experience in virtually all EM methods. Equipment includes Philips CM120, CM12 and CM10 transmission electron microscopes and an ETEC Autoscan scanning electron microscope, a Balzers BAF400T freeze-etching system; three Reichert Ultracut E cryo-ultramicrotomes, and other equipment for specimen preparation. Techniques include immuno-EM, cryo-EM, and preparation of specimens by conventional thin sectioning, negative staining, metal shadowing, cryoultramicrotomy, rapid freezing, freeze substitution, freeze-fracture/freeze-etch, and critical point drying. Dr. Hendricks, aided by an assistant, can provide all services from specimen preparation to printing of electron micrographs. Alternatively, investigators may use Facility reagents and equipment to carry out their own specimen preparation and examination, with assistance from Facility staff as needed. Consequently, the Facility works just as well for users with no EM experience as for those with considerable EM experience. By centralizing all aspects of EM in this facility, the DERC provides its members with the best possible EM services in the most cost- and time-effective manner. The Facility, which is open to all UMMS researchers, is supported by a combination of institutional funds and user chargebacks; DERC investigators are charged at one-half the rate for non-affiliated UMMS researchers. Many DERC members use EM as an integral part of their research, and DERC members have published numerous papers that report results obtained in the EM Core Facility.

DERC 电子显微镜 (EM) 核心设施的目标是为 UMMS 的 DERC 研究人员提供完整、一流的 EM 服务。该设施位于基础和临床科学大楼地下室新装修的套房的中心位置。它是由博士指导的。 George Witman 和 Roger Craig，并由 Gregory Hendricks 博士负责日常管理，Gregory Hendricks 是一位全职专业电子显微镜师，在几乎所有 EM 方法方面拥有丰富的经验。设备包括Philips CM120、CM12和CM10透射电子显微镜和ETEC Autoscan扫描电子显微镜、Balzers BAF400T冷冻蚀刻系统；三台 Reichert Ultracut E 冷冻超薄切片机以及其他用于样品制备的设备。技术包括免疫电镜、冷冻电镜和传统薄层电镜制备标本 切片、负染色、金属遮蔽、冷冻超薄切片、快速冷冻、冷冻替代、冷冻断裂/冷冻蚀刻和临界点干燥。 Hendricks 博士在助手的协助下，可以提供从标本制备到电子显微照片打印的所有服务。或者，研究人员可以根据需要在设施工作人员的协助下，使用设施试剂和设备进行自己的标本制备和检查。因此，该工具对于没有 EM 经验的用户和具有丰富 EM 经验的用户来说都同样有效。通过将 EM 的所有方面集中在该设施中，DERC 以最具成本效益和时间效益的方式为其成员提供最好的 EM 服务。该设施向所有 UMMS 研究人员开放，由机构资金和用户退款共同支持； DERC 研究人员的收费是非附属 UMMS 研究人员的一半。许多 DERC 成员将 EM 作为其研究的一个组成部分，并且 DERC 成员发表了许多论文，报告了 EM 核心设施中获得的结果。