BACTERIAL STUDIES

细菌研究

• 批准号: 7355285
• 负责人: DAVID L POPHAM
• 金额: $ 0.09万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355285
• 关键词: BACTERIAL; STUDIES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacillus subtilis Projects Polymerization of the bacterial peptidoglycan cell wall has proven to be one of the best targets for antibiotics because it is essential for the cells, is highly conserved across essentially all bacterial genera, and takes place outside of the cell, where the antibiotics have easy access. A greater understanding of the details of peptidoglycan polymerization will contribute to the development of new antibiotics. With funding from a grant from the NIH, we study many aspects of peptidoglycan synthesis in B. subtilis. These include: 1) The roles played by the 16 different penicillin-binding proteins (PBPs) in putting together vegetative cell walls and spore cell walls of the proper shape, size and structure; 2) The protein-protein interactions made by the major PBPs; 3) Regulation of the timing of spore peptidoglycan synthesis; and 4) The relationship of spore peptidoglycan structure and spore resistance properties. Bacillus anthracis Projects The spores that cause Anthrax can lie in a dormant, extremely stable state for many years. In addition, these spores are highly resistant to most treatments commonly used for disinfection. Once the spores enter a host body, they must germinate and return to a growing state in order to cause disease. This germination also renders the spores sensitive to disinfection agents. With funding from a grant from the NIH, we are determining the enzymatic activities required for degradation of the B. anthracis spore peptidoglycan wall, an essential step in spore germination. With an understanding of the proteins involved in germination and the mechanism that triggers this process, we may be able to design ways to either prevent or stimulate germination. This can lead to better methods of infection control and cleanup of contaminated sites.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。枯草芽孢杆菌项目 细菌肽聚糖细胞壁的聚合已被证明是抗生素的最佳靶点之一，因为它对细胞至关重要，在基本上所有细菌属中高度保守，并且发生在细胞外，抗生素在细胞外发生方便访问。更好地了解肽聚糖聚合的细节将有助于新抗生素的开发。在美国国立卫生研究院 (NIH) 的资助下，我们研究了枯草芽孢杆菌中肽聚糖合成的许多方面。其中包括： 1) 16 种不同的青霉素结合蛋白 (PBP) 在将营养细胞壁和孢子细胞壁组合成适当的形状、大小和结构方面发挥的作用； 2）主要PBPs产生的蛋白质-蛋白质相互作用； 3）孢子肽聚糖合成时间的调控； 4)孢子肽聚糖结构与孢子抗性特性的关系。 炭疽芽孢杆菌项目 引起炭疽病的孢子可以处于休眠、极其稳定的状态多年。此外，这些孢子对大多数常用的消毒处理方法具有高度抵抗力。一旦孢子进入宿主体内，它们必须发芽并恢复生长状态才能引起疾病。这种萌发还使得孢子对消毒剂敏感。在美国国立卫生研究院 (NIH) 的资助下，我们正在确定降解炭疽芽孢杆菌孢子肽聚糖壁所需的酶活性，这是孢子萌发的重要步骤。通过了解与发芽有关的蛋白质以及触发该过程的机制，我们也许能够设计出阻止或刺激发芽的方法。这可以带来更好的感染控制和污染场地清理方法。