VISION RESEARCH INFRASTRUCTURE DEVELOPMENT GRANT

愿景研究基础设施发展补助金

• 批准号: 7415060
• 负责人: ANTHONY BART NESBURN
• 金额: $ 22.75万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7415060
• 关键词: Arts; California; Cells; Collaborations; Confocal Microscopy; Core Facility; Cultured Cells; Development; Discipline; Disease; Endotoxins; Engineering; Environment; Equipment; Funding; Future; Goals; Grant; Growth; Image; Image Analysis; Immunologist; Individual; Interdisciplinary Study; Laser Scanning Confocal Microscopy; Light; Microscopic; Microscopy; Molecular; Monitor; Mycoplasma; Ophthalmology; Research Infrastructure; Research Personnel; Resources; Scientist; Services; Technology; Time; Universities; Vision; Vision research; cell bank; digital imaging; in vivo; interest; morphometry; new technology; tissue culture

DESCRIPTION (provided by applicant): A Vision Research Infrastructure Development Grant to support vision research within the Department of Ophthalmology at the University of California, Irvine is proposed to provide funding of shared equipment and resources and to promote greater collaboration between vision scientists. Recent growth within the Department has brought together cellular and molecular biologists, immunologists, virologists and engineers that share an interest in studying vision related disease mechanisms. The goals of this proposal are two fold: (1) To bring commonly used technologies together within a shared environment to provide a more efficient mechanism for supporting existing equipment and expertise. (2) To bring together individual investigators from diverse disciplines to provide an environment for collaborative multidisciplinary research that maximally utilizes the individual capabilities of the investigators within the Department of Ophthalmology. In order to achieve these goals we have identified two key functions, (1) Cell Culture; and 2) Microscopic Imaging, that not only need current support but will also greatly expand our abilities to answer vision related questions in the future. We therefore propose to establish two Core Facilities. First, the Cell Culture Facility will be established to a) maintain shared tissue culture equipment and services, b) provide routine cell culture services, c) cell banking, d) mycoplasma and endotoxin monitoring, and e) Vi-CELL(tm) Viability Analyzing. Secondly, the Microscopy Imaging Facility that will provide state-of-the-art services in a) light and fluorescent microscopy, b) confocal laser scanning microscopy, c) multiphoton confocal microscopy and time lapse imaging, d) in vivo confocal microscopy and e) quantitative morphometry and digital image analysis. While the immediate goal of this Infrastructure Development Grant is to establish more centralized facilities to save time, energy and resources, we believe that establishing these facilities will greatly expand the individual capabilities of each investigator through enhanced collaboration with other investigators from different disciplines and by providing easier access to state-of-the-art equipment and the expertise needed to fully use these new technologies.

描述（由申请人提供）：提议提供一项视觉研究基础设施发展拨款，以支持加州大学欧文分校眼科内的视觉研究，为共享设备和资源提供资金，并促进视觉科学家之间加强合作。该部门最近的发展汇集了细胞和分子生物学家、免疫学家、病毒学家和工程师，他们对研究视觉相关疾病机制有着共同的兴趣。该提案的目标有两个：(1) 将常用技术整合到一个共享环境中，为支持现有设备和专业知识提供更有效的机制。 (2) 将来自不同学科的个体研究者聚集在一起，为多学科合作研究提供一个环境，最大限度地利用眼科内研究者的个人能力。为了实现这些目标，我们确定了两个关键功能，（1）细胞培养； 2）显微成像，不仅需要当前的支持，而且还将极大地扩展我们未来回答视觉相关问题的能力。因此，我们建议建立两个核心设施。首先，将建立细胞培养设施，以 a) 维护共享的组织培养设备和服务，b) 提供常规细胞培养服务，c) 细胞库，d) 支原体和内毒素监测，以及 e) Vi-CELL(tm) 活力分析。其次，显微镜成像设施将提供最先进的服务：a）光学和荧光显微镜，b）共焦激光扫描显微镜，c）多光子共焦显微镜和延时成像，d）体内共焦显微镜和e ）定量形态测量和数字图像分析。虽然这项基础设施发展拨款的近期目标是建立更集中的设施以节省时间、精力和资源，但我们相信，建立这些设施将通过加强与来自不同学科的其他研究人员的合作以及提供更容易获得最先进的设备以及充分利用这些新技术所需的专业知识。