Proteomic Studies in Inclusion Body Myositis

包涵体肌炎的蛋白质组学研究

• 批准号: 7468103
• 负责人: STEVEN A GREENBERG
• 金额: $ 20.62万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7468103
• 关键词: Affect; Autoimmunity; Biomedical Research; Data; Disease; Fractionation; Gene Expression; Genetic Transcription; Goals; Inclusion Bodies; Inclusion Body Myositis; Injury; Liquid Chromatography; Mass Spectrum Analysis; Methods; Muscle; Myositis; Nature; Painless; Pathogenesis; Pathway interactions; Patients; Peptides; Process; Proteins; Proteomics; Public Health; Range; Research; Research Personnel; Sampling; Skeletal system; Specimen; Technology; Testing; protein aggregate; protein aggregation; protein expression; tandem mass spectrometry

DESCRIPTION (provided by applicant): Inclusion body myositis (IBM) is a disease of unknown cause characterized by slowly progressive painless weakness and with no effective treatment. It is one of a group of disorders known as inflammatory myopathies, in which some aspect of autoimmunity is believed to be prominent. IBM has been characterized as a disease in which there is accumulation of abnormal proteins. We propose detailed and large-scale studies of proteins in muscle in IBM and other inflammatory myopathies using powerful protein fractionation and mass spectrometry methods. The specific aims are to look broadly at the profile of proteins present within muscle across the range of inflammatory myopathies; to attempt to purify insoluble protein aggregates from IBM muscle and characterize these aggregates by the use of mass spectrometry; and to correlate proteomic with microarray data. PUBLIC HEALTH RELEVANCE. Diseases of protein aggregation are important public health concerns and this research has the potential to further understand the nature of a disease in which protein aggregation is an important part of the mechanism. The technology developed in this proposal has relevance across a wide range of biomedical research.

描述（由申请人提供）：包容体肌炎（IBM）是一种未知原因的疾病，其特征是缓慢进行性无痛和没有有效治疗。它是一组被称为炎症性肌病的疾病之一，其中自身免疫的某些方面被认为是突出的。 IBM被认为是一种疾病，其中存在异常蛋白质。 我们使用强大的蛋白质分馏和质谱法提出了IBM肌肉和其他炎症性肌病中蛋白质的详细和大规模研究。具体的目的是广泛地研究肌肉中肌肉中存在的蛋白质的特征。试图从IBM肌肉中纯化不溶性蛋白质聚集体，并通过使用质谱法来表征这些聚集体；并将蛋白质组学与微阵列数据相关联。公共卫生相关性。蛋白质聚集的疾病是重要的公共卫生问题，这项研究有可能进一步了解蛋白质聚集是该机制重要组成部分的疾病的性质。该提案中开发的技术在广泛的生物医学研究中具有相关性。