UKY DENTAL COBRE: ORAL INFECTIONS: VIRAL INFECTIONS IN CHRONIC ORAL DISEASES

英国牙科 COBRE：口腔感染：慢性口腔疾病中的病毒感染

• 批准号: 7382119
• 负责人: Mengtao Li
• 金额: $ 23.02万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382119
• 关键词: UKY; DENTAL; COBRE; ORAL; INFECTIONS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although bacteria are the primary pathogens in periodontitis, human herpesviruses have a purported relationship with inflamed periodontal tissues. This could be important because herpesviruses modulate host immune responses through a variety of mechanisms. The goal of this research was to test the hypothesis that herpesviruses enhance the pathogenesis of periodontal diseases. Our hypothesis is being examined using three approaches: 1) determine the role of herpesviruses in periodontitis by analyzing clinical samples from patients with and without periodontal disease, 2) investigating the ability of herpesvirus to enhance the progression of periodontitis in the rat model; and 3) define mechanisms of immunomodulation by cytomegalovirus (CMV). We have initiated a plan to recruit 100 patients with periodontal disease and collect dental plaques and saliva samples. Real-time PCR will be used to detect DNA of human herpesvirus in plaque from healthy and diseased sites and parallel saliva samples. This method that has been successfully used to identify all 8 HHVs in saliva is being optimized to detect viral DNA in plaque. In the second project, we have obtained rat CMV (RCMV) Maastricht strain, propagated lab stocks and obtained titers on the virus. Animal inoculation(s) with P. gingivalis and/or RCMV will be initiated in early summer 2005. In the study of molecular mechanisms of immunomodulation by CMV, we have successfully constructed and purified a recombinant CMV that expresses GFP (green fluorescence protein). In addition, we cloned CMV vIL-10 gene. We discovered 5 previously unidentified vIL-10 isoforms. We are in the process of characterizing 8 different vIL-10 isoforms in vitro and analyzing their amino acid sequences using bioinformatics methods. Because CMV vIL-10 is a potent immune suppressor, we are initiating study to determine its roles in modulating the host response of oral epithelia and gingival fibroblasts against bacterial invasion.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。尽管细菌是牙周炎中的主要病原体，但人疱疹病毒与发炎的牙周组织有着关系。这可能很重要，因为疱疹病毒通过多种机制调节宿主免疫反应。这项研究的目的是检验疱疹病毒增强牙周疾病发病机理的假设。我们的假设正在使用三种方法进行检查：1）通过分析和没有牙周疾病患者的临床样本来确定疱疹病毒在牙周炎中的作用，2）研究疱疹病毒在大鼠模型中增强牙周炎进展的能力； 3）定义巨细胞病毒（CMV）的免疫调节机制。我们已经制定了一项计划，招募100例牙周疾病患者并收集牙菌斑和唾液样本。实时PCR将用于检测健康和患病部位和平行唾液样品的斑块中人疱疹病毒的DNA。该方法已成功用于鉴定唾液中的所有8种HHV，以检测斑块中的病毒DNA。在第二个项目中，我们获得了大鼠CMV（RCMV）Maastricht菌株，传播实验室储备并获得了该病毒的滴度。具有牙龈疟原虫和/或RCMV的动物接种将在2005年初启动。在CMV通过免疫调节的分子机制研究中，我们成功地构建并纯化了一种表达GFP的重组CMV（绿色荧光蛋白）。另外，我们克隆了CMV VIL-10基因。我们发现了5个以前未识别的VIL-10同工型。我们正在体外表征8种不同的VIL-10同工型，并使用生物信息学方法分析其氨基酸序列。由于CMV VIL-10是一种有效的免疫抑制剂，因此我们正在启动研究，以确定其在调节口服上皮菌和牙龈成纤维细胞对细菌侵袭的宿主反应中的作用。