MISSISSIPPI COBRE: CORE C: IN VITRO PHARMACOLOGY CORE

密西西比 COBRE：核心 C：体外药理学核心

• 批准号: 7382243
• 负责人: SEAN M WILSON
• 金额: $ 31.73万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-04 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382243
• 关键词: biological products; electrophysiology; gait; housing; lead; pharmacology; receptor; university

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives: The scientific exploration of natural products for the development of psychotropic therapies is an understudied area of research. The In Vitro Pharmacology Core will provide several functions related to understanding the mechanisms of actions of psychoactive natural products that can lead to the development of new therapies. Specifically, Core C will provide the ability in house to screen compounds for biological activity and determine the site(s) as well as mechanism(s) of action. These core functions are dependent on the use of state of the art technologies as well as time-tested laboratory methodologies. Using multi-label reader technologies, the core will be able to rapidly assess compounds and extracts held in the repositories at the University of Mississippi for specific receptor activities. Mechanistic studies into the actions of lead compounds will be assessed using cellular electrophysiology and imaging techniques. Receptor affinity determinations and kinetic analyses will round out Core C's repertoire of techniques. Table 1 presents an overview of the assays that are to be run routinely.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。目标：用于开发精神药物的天然产物的科学探索是一个尚未得到充分研究的研究领域。体外药理学核心将提供与了解精神活性天然产物的作用机制相关的多种功能，从而促进新疗法的开发。具体来说，Core C 将提供内部筛选化合物的生物活性并确定作用位点和机制的能力。这些核心功能取决于最先进的技术以及经过时间考验的实验室方法的使用。使用多标签读取器技术，该核心将能够快速评估密西西比大学存储库中的化合物和提取物的特定受体活性。将使用细胞电生理学和成像技术评估先导化合物作用的机制研究。受体亲和力测定和动力学分析将完善 Core C 的全部技术。表 1 概述了常规运行的测定。