INTERACTIONS BETWEEN PATHOGENS AND ARTHROPOD MIDGUT EPITHELIAL CELLS

病原体与节肢动物中肠上皮细胞之间的相互作用

• 批准号: 7381864
• 负责人: ROLLIE J CLEM
• 金额: $ 2.84万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381864
• 关键词: INTERACTIONS; BETWEEN; PATHOGENS; ARTHROPOD; MIDGUT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptosis is a type of programmed cell death that has been shown to be an important component of innate immunity. When cells become infected with a virus, they often commit suicide by initiating apoptosis, in an attempt to limit the infection. The central hypothesis of this project is that apoptosis can play a role in defense against microbial infection in insect midgut epithelium. This hypothesis was tested under the following specific aims: 1) determine whether apoptosis is stimulated by viral infection in insect midgut epithelial cells, and 2) characterize the apoptotic pathways induced by virus infection in insect cells. In the past year, baculovirus infection of insect midgut epithelium was examined by confocal microscopy. In addition, the mechanisms by which insect cell apoptosis are regulated were defined in greater detail. Studies were also initiated to examine apoptosis in the midgut of mosquitoes infected with Sindbis virus. These results will lead to an increased understanding of apoptosis regulation and its role in anti-viral innate immunity in insects.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。凋亡是一种程序性细胞死亡，已被证明是先天免疫的重要组成部分。 当细胞感染病毒时，他们通常通过造成凋亡来自杀，以限制感染。该项目的中心假设是凋亡可以在防御昆虫中肠上皮中的微生物感染中发挥作用。该假设在以下特定目的下进行了检验：1）确定昆虫中肠中皮细胞中的病毒感染刺激了凋亡，以及2）表征昆虫细胞中病毒感染引起的凋亡途径。在过去的一年中，通过共聚焦显微镜检查了昆虫中肠上皮的杆状病毒感染。另外，更详细地定义了昆虫细胞凋亡的机制。还开始了研究，以检查感染了信德比斯病毒的蚊子中肠中的凋亡。这些结果将导致人们对凋亡调节的了解及其在昆虫抗病毒先天免疫中的作用。