PROTEOMICS STUDIES OF AIRWAY INFLAMMATION

气道炎症的蛋白质组学研究

• 批准号: 7378726
• 负责人: SANJIV SUR
• 金额: $ 0.09万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7378726
• 关键词: PROTEOMICS; STUDIES; AIRWAY; INFLAMMATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background - Asthma is regulated by signaling proteins in the lung. Hypothesis - The signaling proteins in asthma can be identified with new sophisticated laboratory techniques. Specific Aims and Procedures (summary) - To examine the effects of allergen challenge on the changes in proteins present in the lungs for asthmatic and normal subjects. Experimental Design (summary) - Perform bronchoscopy to obtain tissue and fluid from the lung after an inhalation challenge. Tissue, fluid, and blood will be analyzed for differences in proteins. The overall goal is to identify important signaling proteins that regulate allergen-induced inflammation, mucus secretion and airway hyperreactivity in asthmatic patients. These studies will validate the usefulness of ICAT (isotope-coded affinity tag) technology for quantitative proteomics of posttranslationally modified signaling molecules. Further it will develop a signaling proteome for asthmatic airways.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。背景简介 - 哮喘由肺部信号蛋白调节。 假设 - 哮喘中的信号蛋白可以通过新的复杂实验室技术来识别。 具体目标和程序（摘要） - 检查过敏原激发对哮喘和正常受试者肺部蛋白质变化的影响。 实验设计（摘要）-在吸入挑战后进行支气管镜检查以从肺部获取组织和液体。将分析组织、体液和血液中蛋白质的差异。 总体目标是确定调节哮喘患者过敏原诱导的炎症、粘液分泌和气道高反应性的重要信号蛋白。这些研究将验证 ICAT（同位素编码亲和标签）技术对于翻译后修饰信号分子定量蛋白质组学的有用性。此外，它将开发哮喘气道的信号蛋白质组。