INTESTINAL ENDOGENOUS ZINC: ROLE FOR METALLOTHIONEIN

肠内源性锌：金属硫蛋白的作用

• 批准号: 7377788
• 负责人: NANCY F KREBS
• 金额: $ 2.6万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7377788
• 关键词: INTESTINAL; ENDOGENOUS; ZINC; ROLE; METALLOTHIONEIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Zinc (Zn) deficiency has been amply demonstrated on a global basis, as have the adverse implications of Zn deficiency on health. Zn has many important functions in the body, and yet much remains to be learned about exactly how Zn is used. Healthy individuals balance the amount of Zn in their bodies by regulating absorption of Zn from food into the gut and by controlling the release of zinc back into the intestine for excretion in feces. Most research has focused on zinc absorption by the gut. This study will better characterize the physiology of and factors affecting zinc secretion into the intestine. An improved understanding of the ways in which the body usually achieves proper zinc balance may help to ultimately treat and/or prevent Zn deficiency. This study will investigate if a protein, metallothionein, controls Zn secretion into the intestine. Based on experimental data from animal studies and our own preliminary data, we hypothesize that the amount of metallothionein in secretions from the pancreas into the small intestine is directly related to the amount of Zn in these secretions. This study will also evaluate whether the amount of metallothionein in secretions from the pancreas changes when the amount of zinc absorbed from food changes and when the Zn load in the body changes. Healthy adults aged 20-50 will be recruited from the Denver metro area. We will study 10 subjects on a diet with Zn levels similar to their usual diets. Subjects will then be randomly assigned to a Zn restricted or supplemented diet. Before and after they begin their assigned diet, subjects will spend 2 days in the GCRC, where a small tube will be inserted into each subject's mouth and advanced down his/her throat to his/her intestine. This tube will be used to suck out secretions that we will then analyze. All subjects will also receive some special forms of Zn on different days in the study. These will help us to measure Zn amounts in the secretions collecte

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。锌（Zn）缺乏症在全球基础上得到了充分的证明，锌缺乏对健康的不利影响也是如此。 Zn在体内具有许多重要的功能，但是关于如何确切使用Zn的使用仍有许多待了解。健康的个体通过调节锌从食物中的吸收和控制锌的释放回肠道以在粪便中排泄来平衡其体内锌的量。大多数研究都集中在肠道上的锌吸收上。这项研究将更好地表征影响锌分泌到肠道的因素和因素。对人体通常达到适当的锌平衡方式的改进理解可能有助于最终治疗和/或预防锌不足。这项研究将调查蛋白质金属硫蛋白是否将锌分泌控制为肠道。基于动物研究和我们自己的初步数据的实验数据，我们假设从胰腺分泌物到小肠中的分泌物中的金属硫蛋白量直接与这些分泌物中的Zn数量有关。这项研究还将评估胰腺分泌物中的金属硫蛋白量是否会发生变化，而当食物变化中吸收的锌含量以及体内的锌负荷发生变化时。 20-50岁的健康成年人将从丹佛都会区招募。我们将研究10个受Zn水平类似于其通常饮食的饮食的受试者。然后，受试者将被随机分配到受Zn限制或补充饮食中。在开始分配的饮食饮食之前和之后，受试者将在GCRC上度过2天，在那里，将插入一个小管中，并将其喉咙插入他/她的肠道。该管将用于吸收我们将分析的分泌物。在研究的不同日子，所有受试者还将获得一些特殊形式的Zn。这些将帮助我们测量分泌物收集的Zn金额