Laboratory

实验室

基本信息

批准号：
7270410
负责人：
Byron Edward Batteiger
金额：
$ 11.56万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7270410
关键词：
antiinfective agents biomedical facility cervical /vaginal smear communicable disease control communicable disease diagnosis communicable disease transmission cooperative study disease /disorder etiology genotype human genetic material tag human tissue polymerase chain reaction sample collection sexually transmitted diseases topical drug application urine women&apos s health

项目摘要

The objective of this core is to provide diagnostic services, patient specimens and laboratory data in support of the projects within the center. The core is comprised of the director, the laboratory manager, and three research analysts. At each patient encounter, various specimens will be obtained and transported to the laboratory for testing, shipping and aliquot archiving. Specimens to be collected include vaginal swabs, urines, endocervical swabs, serum and lesion swabs. Among diagnostic assays that will be performed are polymerase chain reaction assays for C. trachomatis, N. gonorrhoeae, T. vaginalis and M. genitalium. In addition, vaginal swab specimens that contain C. trachomatis DNA will be processed for chlamydial strain genotyping by nucleotide sequencing of the omp1 gene, and for quantitative estimation of organism load by PCR. Fresh clinical isolates of herpes simplex virus types 1 and 2 will be obtained from subjects in the clinical core and provided to Dr. Spear for use in her mouse model of HSV genital infection. In addition, endocervical samples from women with and without N. gonorrhoeae infection will be provided for detection of gonococcal phospholipase D.

该核心的目的是提供诊断服务，患者标本和实验室数据支持中心内的项目。核心由董事，实验室经理和三位研究分析师组成。在每个患者遇到的情况下，将获得各种标本，并将其运送到实验室进行测试，运输和等分试样归档。要收集的标本包括阴道拭子，尿液，肠托拭子，血清和病变拭子。在将要进行的诊断测定中，有聚合酶链反应测定法，促分藻菌，淋病，阴道链球菌和生殖器。此外，将通过核苷酸测序OMP1基因的核苷酸测序以及PCR对生物体负荷进行定量估计，将处理包含沙眼梭状芽胞盘DNA的阴道拭子标本来处理衣原体菌株基因分型。单纯疱疹病毒1和2的新鲜临床分离株将从临床核心的受试者中获得，并提供给Spear博士，以供其HSV生殖器感染的小鼠模型中使用。此外，还将提供来自有或没有淋病猪笼草的妇女感染的妇女的宫颈样品，以检测淋球菌磷脂酶D。