Laboratory

实验室

基本信息

批准号：
6847210
负责人：
Byron Edward Batteiger
金额：
$ 10.74万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-08-01 至 2009-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6847210
关键词：
antiinfective agents biomedical facility cervical /vaginal smear communicable disease control communicable disease diagnosis communicable disease transmission cooperative study disease /disorder etiology genotype human genetic material tag human tissue polymerase chain reaction sample collection sexually transmitted diseases topical drug application urine women&apos s health

项目摘要

The objective of this core is to provide diagnostic services, patient specimens and laboratory data in support of the projects within the center. The core is comprised of the director, the laboratory manager, and three research analysts. At each patient encounter, various specimens will be obtained and transported to the laboratory for testing, shipping and aliquot archiving. Specimens to be collected include vaginal swabs, urines, endocervical swabs, serum and lesion swabs. Among diagnostic assays that will be performed are polymerase chain reaction assays for C. trachomatis, N. gonorrhoeae, T. vaginalis and M. genitalium. In addition, vaginal swab specimens that contain C. trachomatis DNA will be processed for chlamydial strain genotyping by nucleotide sequencing of the omp1 gene, and for quantitative estimation of organism load by PCR. Fresh clinical isolates of herpes simplex virus types 1 and 2 will be obtained from subjects in the clinical core and provided to Dr. Spear for use in her mouse model of HSV genital infection. In addition, endocervical samples from women with and without N. gonorrhoeae infection will be provided for detection of gonococcal phospholipase D.

该核心的目标是提供诊断服务、患者标本和支持中心项目的实验室数据。核心由主任、实验室经理和三名研究分析师组成。每次接触患者时，都会获取各种样本并将其运送到实验室进行测试、运输和等分存档。采集的标本包括阴道拭子、尿液、宫颈内拭子、血清和病变拭子。将进行的诊断测定包括沙眼衣原体、淋病奈瑟菌、阴道毛滴虫和生殖支原体的聚合酶链反应测定。此外，含有沙眼衣原体 DNA 的阴道拭子样本将通过 omp1 基因的核苷酸测序进行衣原体菌株基因分型，并通过 PCR 定量估计微生物负荷。 1 型和 2 型单纯疱疹病毒的新鲜临床分离株将从临床核心受试者中获得，并提供给 Spear 博士用于她的 HSV 生殖器感染小鼠模型。此外，还将提供感染和未感染淋病奈瑟菌的女性的宫颈内膜样本，用于检测淋球菌磷脂酶 D。