Cell Identity and differentiation in stimulus response

刺激反应中的细胞识别和分化

• 批准号: 7318870
• 负责人: JOSE R DINNENY
• 金额: $ 1.94万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2008-04-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7318870
• 关键词: Aftercare; Behavior; Cell Separation; Cells; Complex; Data; Depth; Development; Exposure to; Future; Genes; Genetic; Goals; Image; Imagery; Individual; Lead; Methodology; Monitor; Mouse-ear Cress; Organ; Output; Perception; Phenotype; Physiology; Plant Model; Plant Roots; Plants; Play; Quality Control; Regulation; Research Personnel; Response to stimulus physiology; Role; Signal Transduction; Stimulus; System; Work; base; cell type; cost; design; mutant; new technology; research study; response; tool

Organs are composed of cells with distinct identities designed to interpret stimuli in specialized ways and coordinately fulfill complex functions. In order to understand how cells perform these specialized functions I plan to study the mechanisms by which cell identity alters the quality and quantity of a stimulus response. I propose to use Fluorescent Activated Cell Sorting (FACS) in combination with cell-type specific fluorescent marker lines to isolate individual cell types of the root after treatment with a stimulus. Using microarrays, I will then analyze the transcriptional changes that occur in each cell type of the root and identify genes that demonstrate cell-type specific transcriptional responses. These data will not only lead to a better understanding of the relationship between cell identity and stimulus perception and response but will also lead to the development of markers to monitor this regulation and marker-based mutant screens to identify the genetic mechanisms that control it. In addition, I propose to collaborate in the development of a high-throughput platform for visualizing root development and physiology termed the "root array" that will facilitate the work proposed here and contribute to the design of future experiments.

器官由具有不同身份的细胞组成，旨在以专门的方式解释刺激，并 协调地履行复杂的职能。为了了解细胞如何执行这些特殊功能 我计划研究细胞身份改变刺激反应的质量和数量的机制。 我建议将荧光激活细胞分选 (FACS) 与细胞类型特异性结合使用 荧光标记线用于在刺激处理后分离根部的各个细胞类型。使用 微阵列，然后我将分析根的每种细胞类型中发生的转录变化 识别表现出细胞类型特异性转录反应的基因。这些数据不仅会引领 更好地理解细胞身份与刺激感知和反应之间的关系，但是 还将导致标记物的开发来监测这种调节和基于标记的突变体筛选 确定控制它的遗传机制。此外，我建议合作开发一个 用于可视化根发育和生理学的高通量平台，称为“根阵列”，它将 促进此处提出的工作并为未来实验的设计做出贡献。