The Role of Separase During Cytokinesis in C elegans

分离酶在秀丽隐杆线虫细胞分裂过程中的作用

• 批准号: 7578442
• 负责人: Joshua Nathaniel Bembenek
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7578442
• 关键词: Affect; Alleles; Anaphase; Animals; Behavior; Biological; Caenorhabditis elegans; Cell Division Process; Cell physiology; Cells; Chromosome Segregation; Chromosomes; Condition; Cytokinesis; Cytoplasmic Structures; Defect; Embryo; Endopeptidases; Ensure; Failure; Fright; Genetic; Genetic Materials; Genetic Nondisjunction; Homologous Gene; Laboratories; Learning; Localized; Mechanics; Meiosis; Mitotic; Molecular; Osmotic Pressure; Pathway interactions; Peptide Hydrolases; Range; Role; Saccharomycetales; Signal Pathway; Sister Chromatid; Structure; Techniques; Temperature; Time; cohesion; mutant; separase; transmission process

The process of cell division requires the correct sequence and timing of various cellular processes to ensure faithful transmission of the genetic material and cellular contents. The entry to anaphase occurs upon the cleavage of sister-chromatid cohesion by a protease known as separase. Separase has recently been shown to promote anaphase spindle dynamics and cytokinesis concomitantly with chromosome segregation in budding yeast. Previous studies from our laboratory identified a temperature-sensitive allele of the separase homologue in C. elegans. Embryos from these separase-mutant worms shifted to the restrictive temperature display several meiotic and mitotic defects such as chromosome nondisjunction, osmotic and mechanical sensitivity, and cytokinesis failure. Using various genetic and cell biological techniques, I propose to uncover the mechanisms by which separase contributes to the completion of cytokinesis in the early embryo of C. elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis.

细胞分裂的过程需要各种蜂窝过程的正确顺序和时机，以确保 忠实的遗传物质和细胞含量的传播。后期的输入发生在 通过称为分离酶的蛋白酶对姐妹染色剂的裂解。分离酶最近是 显示出与染色体隔离同时促进后期纺锤体动力学和细胞因子的作用 在萌芽的酵母中。我们实验室的先前研究确定了对温度敏感的等位基因 秀丽隐杆线虫中的分离酶同源物。这些分离酶突变虫的胚胎转移到了限制性 温度显示几种减数分裂和有丝分裂缺陷，例如染色体非分离，渗透和 机械灵敏度和细胞因子衰竭。使用各种遗传和细胞生物学技术，I 建议揭示分离酶有助于完成细胞因子的机制 秀丽隐杆线虫的早期胚胎。通过这种分析，我希望扩展对分子的理解 用于染色体隔离的时间协调的机制与后期的开始 和细胞因子。