CORRELATION OF GLYCAN STRUCTURES WITH GLYCAN-RELATED GENE EXPRESSION

聚糖结构与聚糖相关基因表达的相关性

• 批准号: 7359079
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.37万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359079
• 关键词: CORRELATION; GLYCAN; STRUCTURES; RELATED; GENE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcript levels for various enzymes and proteins involved in murine cellular glycosylation and recognition are being quantitated by a qRT-PCR method that allows for medium-throughput (>700 genes) transcript analysis. Parallel glycan structural data have been obtained by Dr. Ann Dell (Imperial College, UK), who has developed a highly sensitive MS¿based profiling approach for N-linked glycan analysis. The mass spectra have been analyzed by Dr. York to provide a normalized relative quantitation of glycans structures in the tissue samples. We are now comparing the transcript levels for the ¿glycan-related¿ genes with the glycan structures associated with glycoproteins to determine the correlation between the transcript levels and the corresponding glycan structures.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一，该子项目和研究者 (PI) 可能已从其他 NIH 来源获得主要资助，因此可以在其他 CRISP 机构中得到体现。所列出的内容适用于该中心，该中心不一定是研究人员所在的机构，正在通过允许中等通量的 qRT-PCR 方法对参与鼠细胞糖基化和识别的各种酶和蛋白质的转录水平进行定量。 （>700 个基因）转录本分析由 Ann Dell 博士（英国帝国理工学院）获得，他开发了高度灵敏的 MS¿ York 博士对基于 N-连接聚糖分析的分析方法进行了分析，以提供组织样本中聚糖结构的标准化相对定量。聚糖相关¿具有与糖蛋白相关的聚糖结构的基因，以确定转录物水平和相应聚糖结构之间的相关性。