Bioprocessing of genetically engineered filamentous phages to underpin new therapeutic and industrial applications

基因工程丝状噬菌体的生物加工支持新的治疗和工业应用

• 批准号: BB/D521465/1
• 负责人: John Ward
• 金额: $ 30.94万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD521465%2F1
• 关键词: Bioprocessing; genetically; engineered; filamentous; phages

The extensive knowledge of phage (also known as bacteriophages and bacterial viruses) genetics and physiology acquired in the past 50 years makes them an attractive option for development of products for pharmaceutical, diagnostic and nanotechnological applications. Modified phages are showing promise in the battle to defeat infectious and genetic diseases. For example, it has been shown that HIV epitopes displayed on a filamentous phage can elicit T-cell and B-cell responses offering a novel approach for vaccine design for a disease that affects approximately 6 million world-wide. Modified phages can be synthesised in high titres in E. coli and in principle processed by techniques readily applicable to considerable scale-up. Until a recently resurgence of interest in phages as anti-microbial agents there has been little research concern with large-scale processing since a few early studies 30 years ago. For this reason modern biochemical engineering and molecular biology concepts have yet to be brought together to address the issues involved. The proposed study will examine these. As the crisis in antibiotic drug resistance by microorganisms continues to develop it is also possible that phages will have an antibiotic role. Though this is not the primary focus of this research, the biochemical engineering studies proposed will bear on to this related field.

在过去50年中获得的遗传学和生理学的广泛了解（也称为噬菌体和细菌病毒）使它们成为开发用于药物，诊断和纳米技术应用产品的产品的吸引人选择。修改后的噬菌体在战斗中表现出了击败传染病和遗传疾病的希望。例如，已经表明，在丝状噬菌体上显示的艾滋病毒表位会引起T细胞和B细胞反应，为疫苗设计提供了一种新颖的疾病方法，该疾病在全球范围内影响约600万。可以在大肠杆菌中的高滴度中合成修改的噬菌体，原则上可以通过容易适用于大量扩大的技术来处理。直到最近对噬菌体作为抗微生物剂的兴趣兴趣之前，自30年前的几次早期研究以来，对大规模加工的研究很少。因此，现代的生化工程和分子生物学概念尚未汇集在一起​​以解决所涉及的问题。拟议的研究将研究这些。随着微生物抗生素耐药性的危机继续发展，噬菌体也可能具有抗生素作用。尽管这不是这项研究的主要重点，但提出的生化工程研究将遵循这一相关领域。

项目成果

Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing

• DOI: 10.1007/s12257-012-0776-9
• 发表时间: 2013-06-01
• 期刊: BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
• 影响因子: 3.2
• 作者: Branston, Steven D.;Stanley, Emma C.;Keshavarz-Moore, Eli
• 通讯作者: Keshavarz-Moore, Eli