STRUCTURAL STUDIES OF MACROMOLECULES USING SYNCHROTRON RADIATION

利用同步辐射进行大分子结构研究

• 批准号: 7370345
• 负责人: Alfonso Mondragon
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370345
• 关键词: STRUCTURAL; STUDIES; MACROMOLECULES; USING; SYNCHROTRON; STRUCTURAL; STUDIES; MACROMOLECULES; USING; SYNCHROTRON

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal is concerned with the study of several different systems: a) type I DNA topoisomerases; b) chicken brain a-spectrin; c) domain I of the RNA component of RNase P, and d) several DNA binding proteins. The work is based on X-ray crystallography. We have been studying E. coli DNA topoisomerase I and III for a number of years and have proposed a catalytic mechanism. We are now in the process of studying the interactions of topoisomerase I with DNA and also the structure of mutant proteins that could shed light on the details of the reaction. We solved the structure of two repeats of chicken brain a-spectrin in 1997 using data collected at SSRL. We are now studying new crystals that contain three consecutive repeats. The third project is concerned with the structure of domain I of the RNA component of RNase P. This domain is 154 bases long and the crystals diffract to 3.3 ¿¿at a synchrotron source. The final project is concerned with the structure of ZntR, a metal-dependent transcriptional activator of E. coli. The crystals are small and we require synchrotron radiation for MAD phasing.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。该提案与几种不同系统的研究有关：a）I型DNA拓扑异构酶； b）鸡肉脑A-specrin; c）RNase P的RNA成分的结构域I和d）几种DNA结合蛋白。该作品基于X射线晶体学。多年来，我们一直在研究大肠杆菌DNA拓扑异构酶I和III多年，并提出了一种催化机制。现在，我们正在研究拓扑异构酶I与DNA的相互作用以及突变蛋白的结构，这些突变蛋白可以阐明反应的细节。我们在1997年使用SSRL收集的数据解决了1997年鸡大脑A-光谱蛋白的两次重复的结构。我们现在正在研究包含三个连续重复序列的新晶体。第三个项目与RNaseP的RNA成分的域I结构有关。该域长154个碱基，在同步子源处晶体衍射为3.3€»。最终项目与Zntr的结构有关，Zntr是大肠杆菌的金属依赖性转录激活剂。晶体很小，我们需要同步辐射来进行疯狂的相位。