IDENTIFICATION OF TOR INTERACTING PROTEINS

Tor 相互作用蛋白的鉴定

• 批准号: 7420664
• 负责人: MARIA E CARDENAS-CORONA
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420664
• 关键词: Saccharomyces cerevisiae; T lymphocyte; evolution; gene expression; human; immune system; immunosuppression; immunosuppressive; isomerase; microorganism growth; play; protein kinase; proteins; ribosomes; role; toxin; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are studying the Tor signaling cascade, which senses nutrients and regulates gene expression, translation, and ribosome biogenesis and has been conserved over a billion years of evolution from yeast to humans. The Tor proteins are novel protein kinases whose functions are inhibited by the immunosuppressive antifungal drug rapamycin in complex with the prolyl isomerase FKBP12. We focus on the antiproliferative drug rapamycin, which suppresses the immune system by blocking signaling events required for activation of T-lymphocytes. Rapamycin is used to treat and prevent graft rejection in organ transplant recipients. Rapamycin is a product of a soil bacterium and likely plays a role in nature distinct from immunosuppression, possibly as a toxin to inhibit growth of competing microorganisms. Based on this hypothesis, we have analyzed in detail the mechanisms of rapamycin action in the yeast Saccharomyces cerevisiae. We would like to continue our analysis of the TOR proteins in collaboration with the Yeast Resource Center.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。我们正在研究 Tor 信号级联，它感知营养物质并调节基因表达、翻译和核糖体生物合成，并且在从酵母到人类的十亿年进化过程中得到了保守。 Tor 蛋白是新型蛋白激酶，其功能受到免疫抑制抗真菌药物雷帕霉素与脯氨酰异构酶 FKBP12 复合物的抑制。我们重点关注抗增殖药物雷帕霉素，它通过阻断 T 淋巴细胞激活所需的信号事件来抑制免疫系统。 雷帕霉素用于治疗和预防器官移植受者的移植物排斥反应。雷帕霉素是土壤细菌的产物，在自然界中可能发挥着与免疫抑制不同的作用，可能作为抑制竞争微生物生长的毒素。基于这一假设，我们详细分析了雷帕霉素在酿酒酵母中的作用机制。我们希望与酵母资源中心合作继续对 TOR 蛋白进行分析。