TRANSCRIPTION OF CHROMATIN TEMPLATES WITH PURIFIED FACTORS

使用纯化因子转录染色质模板

• 批准号: 7420707
• 负责人: James T. Kadonaga
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420707
• 关键词: DNA directed RNA polymerase; acetylation; chromatin; methylation; proteins; transcription factor; ubiquitin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the project is to establish a reconstituted, in vitro transcription assay utilizing pure chromatin templates and purified transcription factors. In addition to the known factors, additional components appear to be required for this system. One of these components, tentatively called FX1, has been purified through 6 chromatographic columns, and the identification of the proteins composing the FX1 will complete the reconstituted transcription assay. This assay will eventually be used in the mechanistic studies of RNA polymerase II transcription, such as acetylation, methylation, ubiquitinylation, etc.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。该项目的目的是利用纯染色质模板和纯化的转录因子建立一个重构的体外转录测定法。除了已知的因素外，该系统似乎还需要其他组件。这些成分之一（暂定称为FX1）已通过6个色谱柱进行纯化，并且构成FX1的蛋白质的鉴定将完成重构的转录测定。该测定最终将用于RNA聚合酶II转录的机械研究，例如乙酰化，甲基化，泛素化等。