PERFORMANCE OF LIQUID HELIUM ELECTRON CRYOMICROSCOPE (JEOL3000SFF)

液氦电子冷冻显微镜 (JEOL3000SFF) 的性能

• 批准号: 7357763
• 负责人: Wah Chiu
• 金额: $ 1.51万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357763
• 关键词: PERFORMANCE; LIQUID; HELIUM; ELECTRON; CRYOMICROSCOPE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The JEOL3000SFF electron cryomicroscope is a liquid helium microscope opearted at 300 kV and specimen temperature of 4.2K. This instrument is presently dedicated to high resolution single particle image data collection. We have 2 well-trained scientists using this instrument routinely. The instrument has been in high performance mode throughout most of the year. The downtimes have attributable mostly to the dirty objective aperture, camera jam and malfunction of the automated specimen cartiledge loading device. The biolgoical projects include icosahedral virus particles and chaperonin particles which are aimed at 4 ¿ resolution data.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一，该子项目和研究者 (PI) 可能已从其他 NIH 来源获得主要资助，因此可以在其他 CRISP 机构中得到体现。列出的是中心，不一定是研究者的机构 JEOL3000SFF 电子冷冻显微镜是在 300 kV 和样品温度 4.2K 下运行的液氦显微镜。该仪器目前专用于高分辨率单粒子图像数据收集，我们有 2 名训练有素的科学家经常使用该仪器，该仪器全年大部分时间都处于高性能模式，主要归因于物镜孔径脏污。 、相机卡顿和自动标本软骨装载装置的故障生物项目包括针对 4 ¿ 的二十面体病毒颗粒和伴侣蛋白颗粒。分辨率数据。