Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) Supplement

斯坦福-SLAC CryoET 标本制备服务中心 (SCSC) 补充资料

基本信息

批准号：
10265915
负责人：
Wah Chiu
金额：
$ 37.73万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-16 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10265915
关键词：
Academia Advisory Committees Area Award Biocompatible Materials Biomedical Research Budgets Cells Cellular Structures Collaborations Communication Communities Complex Consult Cryo-electron tomography Cryoelectron Microscopy Data Collection Educational Curriculum Educational workshop Electron Microscope Equipment European Evaluation Fluorescence Microscopy Freezing Funding Goals Grant Hour Hydration status In Situ Industry Infrastructure Institution Investigation Ions Laboratories Lifting Macromolecular Complexes Manufacturer Name Methods Molecular Structure Organelles Performance Preparation Procedures Process Protocols documentation Request for Applications Research Research Personnel Resolution Resource Allocation Sampling Scanning Scanning Electron Microscopy Schedule Scientist Selection Criteria Services Slice Specimen Subcellular structure Techniques Technology Thick Tissue Sample Tissues Titan Training Training Activity Work data management experience experimental study imaging modality instrument instrumentation interest member operation pressure programs prototype response structural biology tool

项目摘要

Project Summary Upgrade of Aquilos to Aquilos 2 cryo-focused ion beam scanning electron microscope. In our original proposed budget, we requested ~$1M for a focused ion beam instrument. We did not specify the manufacturer of such instrument (i.e. Thermo Fisher or Zeiss). The recent announcement from Thermo Fisher of the Aquilos 2 (~$1.38M), offers multiple exciting features. It has the capabilities of performing lift-out, slice and view and automated milling with 20+ lamellae in 13 hours of operation. These results suggest that cryoFIB can become an automated procedure on very thick, high-pressure vitrified samples. This would be a highly sought-after capability of our Service Center, because we anticipate that there will be a significant demand if we can begin to work with tissues tens to hundreds of microns in thickness. These performance specifications and the results shown by Thermo Fisher are exciting. If funded, we will also investigate whether Zeiss can offer a similar or superior instrument before we make a purchase decision. Certainly, we will consult with our European colleagues who have the prototypes of the new instrument in terms of its robustness as a user facility as in our Center.

项目摘要 Aquilos升级到Aquilos 2 Cerio opocueno tocuenos扫描电子显微镜。在我们的原始拟议的预算，我们要求约100万美元用于专注的离子光束仪器。我们没有指定这种仪器的制造商（即热费舍尔或蔡司）。 Thermo最近的公告 Aquilos 2（约138万美元）的Fisher提供了多个令人兴奋的功能。它具有表演的功能在操作的13小时内，用20多个薄板抬出，切片，视图和自动铣削。这些结果表明，在非常厚的高压玻璃样品上，Crofofib可以成为一种自动化程序。这将是我们服务中心的备受追捧的能力，因为我们期望在那里如果我们可以开始使用数十至数百微米的组织，将是一个巨大的需求厚度。这些性能规格和Thermo Fisher所显示的结果令人兴奋。如果资助后，我们还将调查蔡司在制作之前是否可以提供类似或优越的工具购买决定。当然，我们将咨询拥有原型的欧洲同事新工具的鲁棒性作为用户设施，就像我们中心一样。