S CEREVISIAE GENERAL TRANSCRIPTION FACTOR TFIID

酿酒酵母通用转录因子TFIID

• 批准号: 7357793
• 负责人: RAYMOND H JACOBSON
• 金额: $ 1.51万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357793
• 关键词: CEREVISIAE; GENERAL; TRANSCRIPTION; FACTOR; TFIID

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription factor TFIID is a multi-subunit complex responsible for targeting and nucleating the assembly of RNA polymerase II and other associated factors to promoter start sites in eukaryotic organisms. It is important for the regulation of gene expression. Mis-regulation of gene expression can cause developmental defects, metabolic disorders, and cancer. Understanding the mechanism of transcriptional initiation is an important goal, and structural information about TFIID would contribute towards this end. The TFIID complex is estimated to contain 1.1-1.2 MDa of protein contributed from 15 individual subunits. Structural information for this large complex has been difficult to obtain due to expression and solubility problems for the isolated subunits and difficulty in obtaining sufficient material. Our goal has been to extend on negative stain electron microscopy studies of the yeast TFIID complex carried out by the group of P. Schultz using Tandem Affinity Purification (TAP-tag) isolated material. We will obtain cryo-EM reconstructions of the TFIID complex. This data will be used in our efforts to obtaining a high-resolution X-ray crystal structure. It would be of particular interest to further characterize the interactions of TFIID with other known basal factors using cryo-EM to further reveal the spatial relationships between the various components of the RNA Polymerase II transcription system at promoters.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。转录因子 TFIID 是一种多亚基复合物，负责将 RNA 聚合酶 II 和其他相关因子的组装靶向并成核到真核生物的启动子起始位点。它对于基因表达的调控很重要。基因表达的错误调节可能导致发育缺陷、代谢紊乱和癌症。了解转录起始机制是一个重要目标，有关 TFIID 的结构信息将有助于实现这一目标。 TFIID 复合物估计含有 1.1-1.2 MDa 的蛋白质，由 15 个单独的亚基贡献。由于分离亚基的表达和溶解度问题以及难以获得足够的材料，很难获得这种大型复合物的结构信息。我们的目标是扩展 P. Schultz 小组使用串联亲和纯化 (TAP-tag) 分离材料对酵母 TFIID 复合物进行的负染色电子显微镜研究。我们将获得 TFIID 复合体的冷冻电镜重建。这些数据将用于我们获得高分辨率 X 射线晶体结构的努力。使用冷冻电镜进一步表征 TFIID 与其他已知基础因子的相互作用，以进一步揭示启动子处 RNA 聚合酶 II 转录系统的各个组件之间的空间关系，将是特别令人感兴趣的。