Retrotransposon virus-like particle assembly

逆转录转座子病毒样颗粒组装

基本信息

批准号：
10066719
负责人：
Sean Beckwith
金额：
$ 6.53万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10066719
关键词：
Amino Acids Antiviral Agents Behavior Biochemical Biological Biological Assay Biology Biophysical Process Biophysics Capsid Capsid Proteins Cells Cellular biology Chimera organism Chimeric Proteins Code Communicable Diseases Complementary DNA Data Dependence Detergents Disease Drug Targeting Electron Microscopy Elements Event Evolution Family Fluorescence Microscopy Fossils Genes Genetic Genetic Transcription Genome Health Human Genome Immunoblotting Influentials Interruption Investigation Knowledge Length Life Cycle Stages Liquid substance Malignant Neoplasms Measures Mechanics Medical Microscopy Mission Modeling Molecular Mutate National Institute of General Medical Sciences Natural Immunity Nerve Degeneration Nuclear Import Nucleic Acids Phase Phenotype Play Prion Diseases Prions Process Property Protein Sequence Analysis Proteins RNA Research Resistance Retroelements Retrotransposition Retrotransposon Retroviridae Retrovirology Reverse Transcription Role Saccharomyces cerevisiae Shapes Signal Transduction Synapses System Testing Therapeutic Time Virus Virus-like particle Work Yeasts expectation fitness gag Gene Products information model innovation insight mammalian genome mutant novel prion hypothesis prion-like sup35 synaptic function transposon/insertion element yeast prion

项目摘要

PROJECT SUMMARY/ABSTRACT Retrotransposons and retroviruses shape genome evolution and impact host fitness. Over 40% of the human genome is comprised of mobile DNA elements. These elements, once considered dormant, molecular fossils, are in fact remarkably influential across a diverse array of medically-relevant processes. Retroelements and domesticated components of mobile elements play important roles in neurodegeneration, cancer, pluripotent cells, innate immunity, and synaptic signaling. The retrovirus-like transposon Ty1 is an informative model for understanding mechanisms employed by retrotransposons and retroviruses across their replication cycles. This proposal leverages the genetic, biochemical, and cell biological toolkit developed for studying Ty1 and combines innovative conceptual frameworks from retrovirology, biophysics, protein biology, and cell biology. The proposed project will identify parameters governing Ty1 Gag’s role in virus-like particle assembly. This will advance our understanding of retroelement replication cycles and establish mechanisms that may be utilized by other disease-relevant retrotransposons, retroviruses, and domesticated capsid genes. In addition, identification of novel mechanisms used by retroelements has the potential to define a new class of antiviral drug targets.

项目摘要/摘要逆转座子和逆转录病毒塑造基因组进化和撞击宿主健身。超过40％的人基因组由移动DNA元素组成。这些元素曾经被认为是休眠的分子化石，实际上，在许多与医学相关的过程中都具有极大的影响力。重新元素和移动元件的驯化组件在神经变性，癌症，多能中起重要作用细胞，先天免疫学和突触信号传导。类似逆转录病毒的转座子Ty1是一个信息的信息模型了解逆转录座和逆转录病毒在复制周期中携带的机制。这提案利用用于研究Ty1的遗传，生化和细胞生物学工具包逆转录病毒学，生物物理学，蛋白质生物学和细胞生物学的创新概念框架。提议项目将确定有关TY1 GAG在病毒样颗粒组件中的作用的参数。这将推动我们的理解重新元素复制周期并建立其他可能被其他可能利用的机制与疾病相关的逆转录座，逆转录病毒和驯化的衣壳基因。另外，识别恢复元素使用的新型机制有可能定义一类新的抗病毒药物靶标。