SYNTHESIS & CHARACTERIZATION OF NEW MACROMOLECULAR CONTRAST AGENTS

合成

• 批准号: 7369042
• 负责人: Robert Charles Brasch
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369042
• 关键词: SYNTHESIS; CHARACTERIZATION; NEW; MACROMOLECULAR; CONTRAST

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Imaging contrast agents (or contrast media) are a category of diagnostic pharmaceuticals for enhancing the image contrast of organs/tissues and for displaying the organ function in medical imaging. Macromolecular contrast agents have found their important role in the characterization of microvascular permeability of tumor vessels, due to their blood-pool characteristics. New dendritic contrast agents with well-defined structure, high degree of size homogeneity, varying molecular weights and polyethyleneglycol (PEG) cores, will be synthesized. Their average molecular weights (MW) and MW distribution will be obtained from mass spectrometry, which info will be highly helpful for the chemical synthesis and for the study of their structure-property relationship. In addition, some new small molecular intermediates, which are attributed to the image contrast, need the structure characterization by mass spectrometry, too. Methods: For different macromolecular derivatives (MW 3,000 - 150,000), MALDI-MS could be used for the measurements of their MW and distributions in MW. For small molecular compounds (MW 500 - 2000), ESI-MS or other approaches may be applicable. Results & Discussion: Mass spectra data will be correlated with the data from physical and imaging measurements, the relationship between chemical structure and physical (or imaging) properties will be sought for this new class of macromolecular contrast agents. During the synthesis, the mass spectra data will be highly helpful in the guidance and optimization of synthetic procedures.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。简介：成像对比剂（或对比介质）是一种诊断药物的类别，用于增强器官/组织的图像对比度以及在医学成像中显示器官功能。大分子对比剂已经发现它们在肿瘤血管的微血管渗透性的表征中，由于其血液池特征。将合成新的树突形成造影剂，具有明确定义的结构，高度均匀性，变化的分子量和聚乙二醇（PEG）核心。它们的平均分子量（MW）和MW分布将从质谱中获得，该信息将对化学合成和研究其结构 - 质体关系非常有帮助。此外，一些归因于图像对比的一些新的小分子中间体也需要质谱法的结构表征。方法：对于不同的大分子衍生物（MW 3,000-150,000），MALDI -MS可用于测量其MW和MW中的分布。对于小分子化合物（MW 500-2000），可能适用ESI -MS或其他方法。结果与讨论：质谱数据将与物理和成像测量的数据相关，化学结构与物理（或成像）特性之间的关系将为这类新型的大分子对比剂寻求。在合成过程中，质谱数据将在合成程序的指导和优化中非常有帮助。