Determining the substrate specificity of ER oxidoreductases

确定 ER 氧化还原酶的底物特异性

基本信息

批准号：
BB/D00764X/1
负责人：
Neil Bulleid
金额：
$ 29.65万
依托单位：
University of Manchester
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2006
资助国家：
英国
起止时间：
2006 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FD00764X%2F1
关键词：
Determining substrate specificity ER oxidoreductases

项目摘要

For cells and tissues to remain healthy they must be able to make proteins and the proteins they make must be able to function correctly. The cell has complex machinery for ensuring that when new proteins are made they are functional and are transported to the correct location, be it within the cell or outside. This project will address the general question of how the cell ensures that proteins are made correctly and adopt the correct shape. Proteins are made as a string of amino acids which coil-up or fold to adopt a characteristic shape or three-dimensional structure. Only one such shape is functional and the cell ensures that this shape is adopted by providing helper proteins or chaperones to aid this process. A family of enzymes that are located within the cell are responsible for ensuring that some proteins are made correctly and adopt the correct shape. However little is known about which proteins these enzymes are able to help or indeed what their exact function is. We will use a newly developed technique to identify the substrate proteins for each of these enzymes and use this information to determine precisely their function during the folding of proteins within the cell. The information gained from this work will help with our attempts to understand what the cell needs to produce proteins. Understanding the requirements for protein folding in more depth will aid us to make rational decisions when we try to produce proteins in a recombinant form for either structural biology studies or for medical uses.

为了使细胞和组织保持健康，它们必须能够生产蛋白质，并且它们制造的蛋白质必须能够正常运行。该单元具有复杂的机械，可确保当制造新蛋白质时，它们是功能性的，并将其运输到正确的位置，无论是在单元格内还是外部。该项目将解决细胞如何确保正确制成蛋白质并采用正确形状的一般问题。蛋白质作为一串氨基酸制成，该氨基酸串起或折叠以采用特征形状或三维结构。只有一种这种形状是功能性的，并且细胞通过提供辅助蛋白或伴侣来帮助此过程来确保采用这种形状。一个位于细胞内部的酶家族负责确保正确制成某些蛋白质并采用正确的形状。但是，这些酶能够提供帮助或实际上是什么功能的蛋白质知之甚少。我们将使用新开发的技术来识别每种酶的底物蛋白质，并使用此信息在细胞内蛋白质折叠过程中精确确定其功能。从这项工作中获得的信息将有助于我们尝试了解细胞产生蛋白质所需的内容。当我们试图以重组形式生产结构生物学研究或医疗用途时，了解蛋白质折叠的需求将有助于我们做出合理的决策。