PROTEIN CONFORMATIONAL CHANGE TRIGGERED BY NI-BCHL-A EXCITATION

NI-BCHL-A 兴奋引发的蛋白质构象变化

• 批准号: 7373141
• 负责人: PETER LESLIE DUTTON
• 金额: $ 0.07万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7373141
• 关键词: PROTEIN; CONFORMATIONAL; CHANGE; TRIGGERED; NI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We plan to study structural changes and kinetic mechanism after photoexcitation of a Ni-BChl-a incorporated into a de-novo maquette. A maquette is thereby a purely synthetic peptide structures with protein flexibility that is tailored at the molecular level to accomplish as wide a variety of chemical, mechanical and electrical functions as displayed by natural proteins, but with a simplicity and robustness uncharacteristic of living matter. From previous studies on the optical spectroscopy of Ni-BChl-a complexes, it is known that a repulsion of the axial ligands occurs after photoexcitation. We would like to use this phototrigger to initiate protein motion in a de-novo protein to study protein conformational dynamics. Ultrafast transient absorption spectroscopy is to be used to further investigate into structural changes occurring after photoexcitation.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。我们计划在将Ni-BCHL-A纳入De-Novo Maquette的光激发后研究结构变化和动力学机制。因此，具有蛋白质柔韧性的纯合成肽结构是在分子水平上量身定制的，以实现自然蛋白显示的各种化学，机械和电函数，但具有简单性和鲁棒性，对生活物质具有不符。从先前关于Ni-BCHL-A复合物的光谱的研究，众所周知，轴向配体在光激发后发生了反应。我们想使用此光晶触发来启动De-Novo蛋白质中的蛋白质运动来研究蛋白质构象动力学。超快瞬态吸收光谱法应用于进一步研究光激发后发生的结构变化。