USE OF REASH FOR EM CONNECTIVITY STUDIES IN THE DROSOPHILA BRAIN

使用 REASH 进行果蝇大脑中的 EM 连接研究

• 批准号: 7358074
• 负责人: S. Lawrence Zipursky
• 金额: $ 0.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358074
• 关键词: USE; REASH; EM; CONNECTIVITY; STUDIES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in assessing whether the biarsenical ligands, in particular ReAsH, can be used for studying neuroanatomy in Drosophila using photoconversion and electron microscopy. In proof of principle analysis we will assess whether a tetracysteine motif modified synaptotagmin protein expressed specically in one identified neuronal subclass in the fly can be used to identify presynaptic profiles. These constructs are already in flies. Should ReAsH/photoconversion work in the fly, we will be able to target expression of modified transgenes to many identified neurons whose detailed patterns of synaptic connectivity are not yet known. These studies would provide an invaluable resource for studying the development and function of neural circuits in the fly brain. We started our collaboration with NCMIR in October 2003, exchanged reagents and protocols with scientists at NCMIR and are currently trouble-shooting staining with ReAsH in fly embryos.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。我们有兴趣评估双砷配体，特别是 ReAsH，是否可用于使用光转换和电子显微镜研究果蝇的神经解剖学。在原理分析证明中，我们将评估在果蝇中一个已识别的神经元亚类中特异性表达的四半胱氨酸基序修饰的突触结合蛋白是否可用于识别突触前特征。这些结构已经在苍蝇中。如果ReAsH/光转换在果蝇中发挥作用，我们将能够将修饰的转基因表达靶向许多已识别的神经元，而这些神经元的突触连接的详细模式尚不清楚。这些研究将为研究果蝇大脑神经回路的发育和功能提供宝贵的资源。我们于 2003 年 10 月开始与 NCMIR 合作，与 NCMIR 的科学家交换试剂和方案，目前正在解决果蝇胚胎中 ReAsH 染色的问题。