FLASH/REASH LABELING OF TETRACYSTEINE-TAGGED PROTEINS IN ZEBRAFISH

斑马鱼中四半胱氨酸标记蛋白的 Flash/ReASH 标记

• 批准号: 7358107
• 负责人: MANFRED AUER
• 金额: $ 0.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358107
• 关键词: FLASH; REASH; LABELING; TETRACYSTEINE; TAGGED

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to become familiar with the technical aspects of labeling zebrafish embryos that express proteins genetically altered to contain a tetracysteine tag. This tag is recognized by the commercially available fluorophores FlAsH-EDT2 and ReAsH-EDT2. Binding of either fluorophore to the tetracysteine tag results in a fluorescent signal that can photoconvert diaminobenzidine into an osmiophilic precipitate.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。该项目的目标是熟悉标记斑马鱼胚胎的技术方面，这些胚胎表达经过基因改造以包含四半胱氨酸标签的蛋白质。该标签可被市售荧光团 FlasH-EDT2 和 ReAsH-EDT2 识别。任一荧光团与四半胱氨酸标签的结合都会产生荧光信号，该信号可以将二氨基联苯胺光转化为嗜锇沉淀物。