DETECTION OF VULNERABLE PLAQUES USING RAMAN, FLUORESCENCE AND REFLECTANCE SPECT

使用拉曼、荧光和反射光谱检测易损斑块

• 批准号: 7357953
• 负责人: OBRAD SCEPANOVIC
• 金额: $ 2.03万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357953
• 关键词: Raman spectrometry; carotid artery; cholesterol; collagen; diagnosis; fluorescence; fluorescence spectrometry; hemoglobin; hemorrhage; human; macrophage; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Vulnerable plaques are characterized by morphological features such as a thin or ulcerated fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We argue for the use of a multi-modal spectroscopic method, comprising Raman, diffuse reflectance, and intrinsic fluorescence spectroscopy, to detect these key morphological markers of vulnerable plaque. A preliminary study was performed in vitro on 17 human carotid artery specimens. Following the acquisition of the spectra, each specimen was histologically evaluated. It was determined that parameters obtained from diffuse reflectance spectroscopy, the hemoglobin concentration and the scattering parameter, can potentially be used to detect intraplaque hemorrhage and foam cells, respectively. A parameter extracted from the intrinsic fluorescence that relates to the amount of collagen in the topmost layers of the tissue can be used to sense the presence of a thin fibrous cap. Finally, a Raman spectroscopy parameter related to the amount of cholesterol and necrotic material can potentially be used to detect a necrotic core. The multi-modal spectroscopy method shows great promise to provide depth-sensitive and complementary morphological information about plaque composition. A larger study is needed to further validate the potential for diagnosis of vulnerable plaque.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。脆弱的斑块的特征是形态学特征，例如薄或溃疡的纤维帽，大坏死核，浅表泡沫细胞和laplaque内出血。我们主张使用多模式光谱法，包括拉曼，弥漫性反射率和固有的荧光光谱，以检测易受伤害斑块的这些关键的形态标记。在17个人颈动脉标本的体外进行了初步研究。在获得光谱后，对每个标本进行了组织学评估。已经确定，可以分别使用从弥漫性反射光谱，血红蛋白浓度和散射参数获得的参数，分别用于检测laplaque内出血和泡沫细胞。从固有荧光中提取的参数与组织最高层中的胶原蛋白量有关，可用于感知薄纤维帽的存在。最后，与胆固醇和坏死材料量有关的拉曼光谱参数可能可用于检测坏死核。多模式光谱法显示出巨大的希望，可以提供有关斑块组成的深度敏感和互补的形态信息。需要进行更大的研究以进一步验证诊断脆弱斑块的潜力。