INTERMEDIARY METABOLISM IN HEART BY NMR

通过 NMR 观察心脏的中间代谢

• 批准号: 7357893
• 负责人: Dean Sherry
• 金额: $ 1.17万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357893
• 关键词: Krebs' cycle; heart; intracellular; liver; metabolism; oxidation; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this NIH project (year 18) is to develop 13C NMR a non-invasive tool for examining intermediary metabolism in intact tissues and to apply such techniques to address several fundamental metabolic questions in isolated hearts and livers. Intermediary metabolism is difficult to study because it involves networks of metabolic pathways in different intracellular compartments. A major emphasis in our earlier studies was to simultaneously measure fluxes through multiple substrate oxidation pathways, anaplerosis, the TCA cycle and exchange reactions in the isolated rat heart.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。该NIH项目（第18年）的总体目标是开发13C NMR一种非侵入性工具，用于检查完整组织中的中介代谢，并应用此类技术来解决孤立心脏和肝脏中的几个基本代谢问题。中介代谢很难研究，因为它涉及不同细胞内室中代谢途径的网络。我们较早的研究中的主要重点是通过多个底物氧化途径，静脉粥样硬化，TCA循环和交换反应在分离的大鼠心脏中同时测量通量。